PMID: 6538812May 1, 1984Paper

Cell synchrony techniques. I. A comparison of methods

Cell and Tissue Kinetics
D J GrdinaR A White

Abstract

Selected cell synchrony techniques, as applied to asynchronous populations of Chinese hamster ovary (CHO) cells, have been compared. Aliquots from the same culture of exponentially growing cells were synchronized using mitotic selection, mitotic selection and hydroxyurea block, centrifugal elutriation, or an EPICS V cell sorter. Sorting of cells was achieved after staining cells with Hoechst 33258. After synchronization by the various methods the relative distribution of cells in G1, S, or G2 + M phases of the cell cycle was determined by flow cytometry. Fractions of synchronized cells obtained from each method were replated and allowed to progress through a second cell cycle. Mitotic selection gave rise to relatively pure and unperturbed early G1 phase cells. While cell synchrony rapidly dispersed with time, cells progressed through the cell cycle in 12 hr. Sorting with the EPICS V on the modal G1 peak yielded a relatively pure but heterogeneous G1 population (i.e. early to late G1). Again, synchrony dispersed with time, but cell-cycle progression required 14 hr. With centrifugal elutriation, several different cell populations synchronized throughout the cell cycle could be rapidly obtained with a purity comparable to mitotic ...Continue Reading

References

Jul 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D J Arndt-Jovin, T M Jovin
Aug 1, 1978·Computers and Biomedical Research, an International Journal·D A JohnstonB Barlogie
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Mar 1, 1971·Experimental Cell Research·D GlickC R McEwen
May 1, 1984·Cell and Tissue Kinetics·R A WhiteT S Johnson

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Citations

Nov 13, 2008·Cytotechnology·Valerie S LebleuCharles E Helmstetter
Mar 6, 2004·Neurotoxicology and Teratology·Helen C GallagherCiaran M Regan
Sep 21, 2001·Biochemical and Biophysical Research Communications·M R JiangJ R Wu
Jul 1, 1987·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·J P FreyerM R Raju
Apr 16, 2013·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Mohammed Mohammed IbrahimJianwu Tang
Oct 27, 2007·The EMBO Journal·Benjamin Barré, Neil D Perkins
May 1, 1984·Cell and Tissue Kinetics·R A WhiteT S Johnson
Aug 1, 1987·Journal of Cellular Physiology·B K BhuyanR J Trzos
Mar 21, 2006·Experimental Eye Research·Sara J LiliensiekChristopher J Murphy
Oct 2, 2007·Radiation Research·George D Wilson, Brian Marples
Oct 21, 2000·Physical Review Letters·T L Closson, M R Roussel
Aug 12, 2010·Journal of Speech, Language, and Hearing Research : JSLHR·Wei TianQian Zheng

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