Cell therapy using articular chondrocytes overexpressing BMP-7 or BMP-10 in a rabbit disc organ culture model

Spine
Yejia ZhangDavid W Anderson

Abstract

Rabbit knee articular chondrocytes overexpressing human growth factors were injected into cultured intervertebral disc explants. Survival of the injected cells and accumulation of extracellular matrix were assessed. To define the utility of cell-based gene delivery approach for repair of the intervertebral disc. Back pain associated with symptomatic disc degeneration is a common clinical condition. Growth factors stimulate disc cell metabolism, but the ideal method for in vivo delivery has not been established. Cells as a vehicle for delivering growth factors to the disc offer potential advantages, including prolonged production of the growth factor within the disc and vital cells to participate in the repair process. New Zealand white rabbit articular chondrocytes transduced with adenovirus expressing human bone morphogenetic protein-7 and green fluorescence protein (GFP) (AdhBMP-7), human bone morphogenetic protein-10 and GFP (AdBMP-10), or GFP alone (AdGFP, as a control) were injected into whole disc explants. Discs were maintained in culture for 1 to 2 months. At the conclusion of the culture periods, cell survival was assessed by fluorescence microscopy and extracellular matrix accumulation was assessed with biochemical me...Continue Reading

References

Mar 1, 1991·Spine·J P ThompsonD S Bradford
Jan 1, 1986·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M T BaylissS Holm
Oct 6, 1994·The New England Journal of Medicine·M BrittbergL Peterson
Apr 1, 1997·BioTechniques·L B CreemersV Everts
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·T C HeB Vogelstein
Jan 12, 1999·Experimental Cell Research·D J AguiarT R Oegema
Jul 20, 1999·Science·S AnnunenL Ala-Kokko
Jun 2, 2000·Journal of Biomechanical Engineering·D M Elliott, L A Setton
May 3, 2001·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·A E BaerL A Setton
Sep 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·Christiana DelloRussoJeffrey S Chamberlain
Oct 18, 2002·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Timothy M Ganey, Hans Joerg Meisel
Aug 20, 2003·The Journal of Bone and Joint Surgery. American Volume·Hongwei ChengTong-Chuan He
Mar 5, 2004·Matrix Biology : Journal of the International Society for Matrix Biology·Jun ChenLori A Setton
Jun 24, 2004·American Journal of Physical Medicine & Rehabilitation·Yejia ZhangEugene J M A Thonar
Jul 3, 2004·Clinical Orthopaedics and Related Research·Farshid GuilakJ Brice Weinberg
Nov 27, 2004·Spine·Jill P G UrbanJeremy C T Fairbank
Nov 27, 2004·Spine·Adam L ShimerJames D Kang
Feb 25, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Osamu SugiyamaJay R Lieberman
Mar 2, 2005·Spine·Anthony WestbrookDenis Calthorpe
Nov 18, 2005·The Spine Journal : Official Journal of the North American Spine Society·D Greg Anderson, Chadi Tannoury

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Citations

Nov 16, 2013·Current Pain and Headache Reports·Hamish T J GilbertStephen M Richardson
Aug 13, 2011·Molecular Pharmaceutics·Dmitriy SheynDan Gazit
Sep 23, 2009·Journal of Neurosurgery. Spine·Michael G Fehlings, Babak Arvin
Dec 21, 2010·Journal of Neurosurgery. Spine·Antoine G TohmehMark D Peterson
Dec 3, 2013·The Spine Journal : Official Journal of the North American Spine Society·Khoi D ThanChia-Ying Lin
Nov 8, 2008·Expert Opinion on Biological Therapy·K K Jain
Nov 8, 2008·Expert Opinion on Biological Therapy·K K Jain
Dec 3, 2014·Advanced Drug Delivery Reviews·S B G BlanquerA A Poot
Apr 13, 2013·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Wang ChaofengRuan Dike
Jan 20, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Chaofeng WangYan Zhang
May 14, 2016·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Fabrice A KüllingJeffrey C Lotz
Jul 29, 2015·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Tao GuRuan Dike
Nov 22, 2019·Current Pharmaceutical Biotechnology·Songfeng ChenHongjian Liu
Jan 3, 2013·Journal of Tissue Engineering and Regenerative Medicine·S OdabasE Piskin
Mar 8, 2021·The Spine Journal : Official Journal of the North American Spine Society·Alex M HollenbergAddisu Mesfin

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