Cell-to-cell communication and cellular environment alter the somatostatin status of delta cells

Biochemical and Biophysical Research Communications
Catriona KellyNeville H McClenaghan

Abstract

Somatostatin, released from pancreatic delta cells, is a potent paracrine inhibitor of insulin and glucagon secretion. Islet cellular interactions and glucose homeostasis are essential to maintain normal patterns of insulin secretion. However, the importance of cell-to-cell communication and cellular environment in the regulation of somatostatin release remains unclear. This study employed the somatostatin-secreting TGP52 cell line maintained in DMEM:F12 (17.5mM glucose) or DMEM (25mM glucose) culture media. The effect of pseudoislet formation and culture medium on somatostatin content and release in response to a variety of stimuli was measured by somatostatin EIA. In addition, the effect of pseudoislet formation on cellular viability (MTT and LDH assays) and proliferation (BrdU ELISA) was determined. TGP52 cells readily formed pseudoislets and showed enhanced functionality in three-dimensional form with increased E-cadherin expression irrespective of the culture environment used. However, culture in DMEM decreased cellular somatostatin content (P<0.01) and increased somatostatin secretion in response to a variety of stimuli including arginine, calcium and PMA (P<0.001) when compared with cells grown in DMEM:F12. Configuration...Continue Reading

References

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Citations

Aug 4, 2015·Journal of Diabetes Research·Lukáš AlánPetr Ježek
Jun 13, 2017·The Journal of Clinical Investigation·Qing LiXiao Yu
Aug 19, 2021·Archives of Microbiology·Soma Ghosh, Sreemanta Pramanik

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