Cell-to-cell movement of potato virus X is associated with a change in the size-exclusion limit of plasmodesmata in trichome cells of Nicotiana clevelandii

Virology
S M AngellD C Baulcombe

Abstract

To study the effect of cell-to-cell movement of potato virus X (PVX) on the size-exclusion limit of plasmodesmata we have microinjected virus particles and fluorescently labelled dextrans (F-dextrans) with average molecular mass of 4.4, 10, and 20 kDa into Nicotiana clevelandii trichome cells. A vector construct of PVX expressing GUS (PVX.GUS) moved from the injected cell and modified the plasmodesmata to allow passage of the 4.4- and 10-kDa F-dextrans but not the 20-kDa F-dextran. A mutant PVX with a deletion in the gene for the 25-kDa protein accumulated in inoculated protoplasts to the same level as the wild-type PVX. This mutant virus did not move from the injected cell and did not modify the plasmodesmata to allow passage of the 10-kDa F-dextran. However, the 4.4-kDa F-dextran moved from the cells infected with the mutant virus. These data therefore illustrate that movement of PVX is associated with modification of plasmodesmata. The full plasmodesmatal modification requires a functional 25-kDa protein but partial modification occurs when this protein is present in a mutant form.

Citations

Jun 27, 2013·The Journal of Cell Biology·Jens TilsnerKarl J Oparka
Apr 15, 2005·Molecular Plant-microbe Interactions : MPMI·Jeanmarie Verchot-Lubicz
Feb 11, 2003·Molecular Plant-microbe Interactions : MPMI·Ingela FridborgSusan Angell
Jun 23, 2011·Phytopathology·P KogovšekM Pompe-Novak
Apr 23, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J G AtabekovN A Shapka
Apr 23, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·V Citovsky
Jul 1, 1997·The Plant Cell·B G McLeanP C Zambryski
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Soumitra GhoshroyVitaly Citovsky
Jun 14, 2006·Annual Review of Microbiology·Feng Li, Shou-Wei Ding
Oct 21, 2009·Virology Journal·Diana L Trejo-SaavedraRafael F Rivera-Bustamante
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·E V RyabovM E Taliansky
Jun 1, 2000·Molecular Plant-microbe Interactions : MPMI·Y YangJ Verchot
Oct 6, 2012·Current Opinion in Virology·Jens Tilsner, Karl J Oparka
May 5, 2012·Progress in Lipid Research·Jean-Luc CacasSébastien Mongrand
Jul 18, 2008·Virus Research·Natalia Andrea MódenaAlejandro Mentaberry
Dec 5, 2015·Frontiers in Plant Science·Chiara LicoSelene Baschieri
Jan 1, 2004·Molecular Plant Pathology·Guillaume Moissiard, Olivier Voinnet
Jun 18, 2004·Immunological Reviews·Charles-Henri Lecellier, Olivier Voinnet
Mar 1, 2003·Molecular Plant Pathology·Jeffrey S BattenCynthia Hemenway
Oct 25, 2011·Molecular Plant Pathology·Karen-Beth G ScholthofGary D Foster
Oct 12, 2004·Plant Physiology and Biochemistry : PPB·Ken KobayashiAlejandro Mentaberry
Dec 14, 2005·Methods : a Companion to Methods in Enzymology·Péter Lénárt, Jan Ellenberg
Feb 13, 2001·The Journal of General Virology·O FedorkinS Yu Morozov
Jan 18, 2013·The Journal of General Virology·M Naeem SattarRob W Briddon
May 8, 2015·Current Opinion in Virology·Ming-Ru LiouYau-Heiu Hsu

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