Cell-to-cell tracer movement in cardiac muscle. Ruthenium red vs. lanthanum

Cell and Tissue Research
K M Baldwin

Abstract

An attempt was made to label injured cardiac muscle cells by exposing them to two electron-opaque tracers, ruthenium red and lanthanum nitrate. To do this, false tendons of sheep hearts containing strands of Purkinje fibers were sectioned, allowed to heal, and then exposed to the tracer during fixation. After this treatment, a group of cells near the cut end were found to be labelled intracellularly with the tracers while the remaining cells in the strand were unlabelled. For comparison, several false tendons were fixed briefly in glutaraldehyde before being cut and then exposed to the tracer. With lanthanum, the results were similar to those obtained when the cells had been damaged prior to fixation. However, when ruthenium red was used as the tracer, it penetrated much further into the cellular strand, its intensity gradually diminishing with distance from the cut end. This finding of apparent dye-coupling in fixed tissue was surprising since it has been suggested that glutaraldehyde fixation converts all communicating junctions to be uncoupled state. Dye-coupling of fixed tissue with ruthenium red as a tracer was seen also in frog atrial trabeculae. Gap junctions between injured (and presumably uncoupled) sheep heart Purkinj...Continue Reading

References

Mar 1, 1979·Cell Biology International Reports·W C De Mello
Dec 1, 1977·Journal of Molecular and Cellular Cardiology·K M Baldwin
Jul 1, 1978·The Journal of General Physiology·P R Brink, M M Dewey
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Feb 1, 1961·The Journal of Biophysical and Biochemical Cytology·J H LUFT

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Citations

Apr 1, 1990·Journal of Electron Microscopy Technique·I S HarperA Lochner
Dec 1, 1985·Pflügers Archiv : European journal of physiology·M F Wendt-Gallitelli, G Isenberg
Aug 1, 1995·Microscopy Research and Technique·N J Severs
Feb 1, 1990·International Journal of Cardiology·N J Severs
Sep 1, 1984·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S W FrenchP S Sun

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