Cell-to-cell transport of movement-defective cucumber mosaic and tobacco mosaic viruses in transgenic plants expressing heterologous movement protein genes

Virology
B CooperJ A Dodds

Abstract

To determine if the movement proteins (MPs) of cucumber mosaic cucumovirus (CMV) and tobacco mosaic tobamovirus (TMV) are complementary in function, transgenic plants expressing genes encoding TMV or CMV MP were inoculated with movement-defective mutants of TMV and CMV. Transgenic plants expressing the MP gene of CMV strain S (subgroup II) complemented the cell-to-cell and systemic spread of a movement-defective mutant of CMV strain Fny (subgroup I) but not the local or systemic spread of a movement-defective mutant of TMV. Plants that contained the MP gene from CMV-S were not resistant to wild-type TMV infection. When inoculated with a movement-defective mutant of TMV that produced beta-glucuronidase, transgenic plants with the CMV MP gene supported only subliminal infection. Conversely, immunodetection and in situ localization techniques revealed that transgenic plants accumulating the TMV MP supported cell-to-cell spread, but not systemic transport, of a movement-defective CMV. These studies suggest that the transgenic TMV MP shares some of the functions with the CMV MP required to transport CMV, whereas the transgenic CMV MP is deficient in functions that are needed to mobilize the spread of TMV infection.

Citations

Sep 23, 1998·The Plant Journal : for Cell and Molecular Biology·T W KahnR N Beachy
Jan 19, 2002·Journal of Virology·Pilar SáenzJuan Antonio García
May 4, 2006·Annual Review of Plant Biology·Tony J Lough, William J Lucas
Mar 9, 2004·Current Opinion in Plant Biology·Yuri GlebaVictor Klimyuk
Aug 19, 2008·Molecular Plant Pathology·Jonathan R Latham, Allison K Wilson
Feb 28, 2007·The Journal of General Virology·Diana Pohl, Christina Wege

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