Cell transplantation strategies for acquired and inherited disorders of peripheral myelin

Annals of Clinical and Translational Neurology
A K M G MuhammadRobert H Baloh

Abstract

To investigate transplantation of rat Schwann cells or human iPSC-derived neural crest cells and derivatives into models of acquired and inherited peripheral myelin damage. Primary cultured rat Schwann cells labeled with a fluorescent protein for monitoring at various times after transplantation. Human-induced pluripotent stem cells (iPSCs) were differentiated into neural crest stem cells, and subsequently toward a Schwann cell lineage via two different protocols. Cell types were characterized using flow cytometry, immunocytochemistry, and transcriptomics. Rat Schwann cells and human iPSC derivatives were transplanted into (1) nude rats pretreated with lysolecithin to induce demyelination or (2) a transgenic rat model of dysmyelination due to PMP22 overexpression. Rat Schwann cells transplanted into sciatic nerves with either toxic demyelination or genetic dysmyelination engrafted successfully, and migrated longitudinally for relatively long distances, with more limited axial migration. Transplanted Schwann cells engaged existing axons and displaced dysfunctional Schwann cells to form normal-appearing myelin. Human iPSC-derived neural crest stem cells and their derivatives shared similar engraftment and migration characteristic...Continue Reading

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Citations

Feb 1, 2019·Expert Opinion on Biological Therapy·Sara Sayad-FathiArash Zaminy
Jun 27, 2020·Stem Cell Research & Therapy·Han-Seop KimYee Sook Cho
Jan 30, 2021·Stem Cells Translational Medicine·Juliane D GlaeserDmitriy Sheyn
Apr 1, 2021·Journal of Biomedical Nanotechnology·Kai YeJiabo Hu

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Methods Mentioned

BETA
transgenic
dissection
light microscopy
flow cytometry
biopsy

Software Mentioned

Partek
Adobe Photoshop
FlowJo
MesenPRO
Graph Pad
BOWTIE
Wand
ImageJ
Prism

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