Cell type composition and circuit organization of clonally related excitatory neurons in the juvenile mouse neocortex.

ELife
Cathryn Rene CadwellA.S. Tolias

Abstract

Clones of excitatory neurons derived from a common progenitor have been proposed to serve as elementary information processing modules in the neocortex. To characterize the cell types and circuit diagram of clonally related excitatory neurons, we performed multi-cell patch clamp recordings and Patch-seq on neurons derived from Nestin-positive progenitors labeled by tamoxifen induction at embryonic day 10.5. The resulting clones are derived from two radial glia on average, span cortical layers 2-6, and are composed of a random sampling of transcriptomic cell types. We find an interaction between shared lineage and connection type: related neurons are more likely to be connected vertically across cortical layers, but not laterally within the same layer. These findings challenge the view that related neurons show uniformly increased connectivity and suggest that integration of vertical intra-clonal input with lateral inter-clonal input may represent a developmentally programmed connectivity motif supporting the emergence of functional circuits.

References

Jul 8, 1988·Science·P Rakic
Sep 1, 1995·Trends in Neurosciences·V S CavinessR S Nowakowski
Mar 1, 1997·Human Reproduction·S R Milligan, C A Finn
Apr 1, 1997·Brain : a Journal of Neurology·V B Mountcastle
Apr 19, 2002·Brain : a Journal of Neurology·Daniel P Buxhoeveden, Manuel F Casanova
May 4, 2002·Nature Reviews. Genetics·Amitabh GuptaAnthony Wynshaw-Boris
Jun 7, 2002·Nature·Kresimir LetinicPasko Rakic
Jun 29, 2004·Trends in Neurosciences·Arnold R Kriegstein, Stephen C Noctor
Jan 7, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Chris EnglundRobert F Hevner
Mar 28, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Bruno PéaultJohnny Huard
Apr 25, 2007·Nature Reviews. Neuroscience·Franck PolleuxAnirvan Ghosh
Apr 8, 2009·Nature Methods·Fuchou TangM Azim Surani
Mar 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Rodrigo PerinHenry Markram
Jan 1, 1993·Methods in Molecular Biology·D Murphy
Jul 17, 2012·Neuron·Gordon B Smith, David Fitzpatrick
Jul 17, 2012·Frontiers in Neuroanatomy·Dirk Feldmeyer
Aug 11, 2012·Science·Santos J FrancoUlrich Müller
Sep 24, 2013·Nature Methods·Simone PicelliRickard Sandberg
Jan 5, 2014·Nature Protocols·Simone PicelliRickard Sandberg
Aug 1, 2014·Genome Research·Simone PicelliRickard Sandberg
May 23, 2015·Neuron·Cristina Gil-SanzUlrich Müller

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Citations

Nov 17, 2020·Frontiers in Computational Neuroscience·Dileep GeorgeJ Swaroop Guntupalli
Feb 2, 2021·Science Advances·Kazuki OkamotoYuji Ikegaya
Nov 24, 2020·Current Opinion in Neurobiology·Yang LinSong-Hai Shi
Oct 24, 2020·Current Opinion in Neurobiology·Alfredo Llorca, Oscar Marín
Mar 18, 2021·American Journal of Physiology. Gastrointestinal and Liver Physiology·Vikram JoshiArthur Beyder

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Datasets Mentioned

BETA
GSE140946

Methods Mentioned

BETA
transgenic
Patch-seq
PCR
genotyping
chip
PCA
RNA-seq

Software Mentioned

STAR
Adobe Photoshop
Adobe Illustrator
Matlab
PatchMaster
scran
correlatePairs
glmfit
R Bioconductor

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