Cell Type Diversity in Hepatitis B Virus RNA Splicing and Its Regulation

Frontiers in Microbiology
Noriomi ItoTetsuro Suzuki

Abstract

Although RNA splicing of hepatitis B virus (HBV) is a commonly observed in livers of hepatitis B patients as well as in the cultured cells replicating the viral genome, its biological significance in the HBV life cycle and the detailed regulatory mechanisms are still largely unclear. In this study, we found cell-type dependency of HBV splicing of the 3.5 kb pregenomic RNA, which is efficiently spliced in human hepatoma cells but not in cells derived from human hepatic stellate, mouse hepatoma and human non-hepatic cells. It may be likely that RNA splicing is one of the determinants of host range restriction of HBV. Given the finding indicating the difference in cell-type dependency of the splicing efficiency between HBV and simian virus 40, we carried out intron-swapping experiments. The results suggest the presence of putative exonic splicing enhancer that possibly works in the cell-type dependent fashion. Together with further mutational analyses, a novel 50-nt intronic splicing silencer, whose secondary structure is well conserved among the HBV strains, was identified. It appears that this intronic silencer functions effectively independent of cell backgrounds.

References

Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T SuzukiT Miyamura
Sep 1, 1989·Journal of Virology·T S SuS H Han
Jan 5, 2000·The Journal of Clinical Investigation·P SoussanD Kremsdorf
Sep 5, 2003·Cell Transplantation·Norikuni ShibataNaoya Kobayashi
May 6, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Fuminaka SugauchiMasashi Mizokami
Jan 18, 2006·Nucleic Acids Research·Tilman HeiseHeiner Schaal
May 30, 2008·Frontiers in Bioscience : a Journal and Virtual Library·Gunhild Sommer, Tilman Heise
Jun 6, 2008·The Journal of Infectious Diseases·Patrick SoussanDina Kremsdorf
Oct 4, 2011·FEBS Letters·Joydip Bhanja ChowdhurySagarmoy Ghosh
Apr 28, 2012·Gastroenterology·Hashem B El-Serag
Jun 6, 2012·Virology·Francois RedelspergerPatrick Soussan
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Jul 3, 2013·Journal of Hepatology·Julianne BaylissPeter A Revill
Jun 18, 2017·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Takeshi ChidaTetsuro Suzuki

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Methods Mentioned

BETA
ISS
PCR
PCRs
transfection
in vitro transcription
electrophoresis
ISSs

Software Mentioned

ImageJ

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