DOI: 10.1101/502773Dec 20, 2018Paper

Cell type purification by single-cell transcriptome-trained sorting

BioRxiv : the Preprint Server for Biology
Chloe S BaronAlexander van Oudenaarden

Abstract

Traditional cell type enrichment using fluorescence activated cell sorting (FACS) relies on methods that specifically label the cell type of interest. Here we propose GateID, a computational method that combines single-cell transcriptomics for unbiased cell type identification with FACS index sorting to purify cell types of choice. We validate GateID by purifying various cell types from the zebrafish kidney marrow and the human pancreas without resorting to specific antibodies or transgenes.

Related Concepts

Pancreas
Zebrafish
Single cell proteins
Fluorescence-Activated Cell Sorting
Transgenes
Marrow
Computed (Procedure)

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