PMID: 11311892Apr 20, 2001Paper

Cell type-specific differences in the coupling of recombinant mGlu1alpha receptors to endogenous G protein sub-populations

Neuropharmacology
J V SelkirkR A Challiss

Abstract

In this study the effects of cell background on the coupling of the type 1alpha metabotropic glutamate (mGlu1alpha) receptor to different G protein sub-populations by recombinant expression of this receptor subtype in baby hamster kidney (BHK) and Chinese hamster ovary (CHO) cells have been investigated. Receptor-G protein interactions were assessed using [(35)S]GTPgammaS binding and subsequent Galpha subunit-specific immunoprecipitation. In a CHO cell line (CHO-lac-mGlu1alpha), where mGlu1alpha receptor expression is under inducible control, stimulation of membranes with the mGlu receptor agonist quisqualate resulted in an increase in specific [(35)S]GTPgammaS binding to G(q/11)alpha only, whereas in a BHK cell line (BHK-mGlu1alpha) agonist stimulation increased [(35)S]GTPgammaS binding to G(q/11)alpha and also to pertussis toxin (PTx)-sensitive G(i/o) proteins (assessed using G(i1/2)alpha- and G(i3/o)alpha-specific antibodies). These data are consistent with our previous observations of dual, antagonistic G(q/11)/G(i/o) regulation of phospholipase C (PLC) in BHK-mGlu1alpha cells, whereas no evidence was found for a G(i/o) modulation of PLC activity in the CHO-lac-mGlu1alpha cell line. PTx pre-treatment of either cell line had...Continue Reading

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Citations

Mar 1, 2005·Visual Neuroscience·Romina Sosa, Evanna Gleason
Jun 20, 2001·Journal of Neurochemistry·M S NashS R Nahorski
Jun 18, 2002·Journal of Neurochemistry·Romina SosaEvanna L Gleason
Jul 5, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Alison L DellJonathan A Raper
Oct 25, 2013·International Journal of Biological Sciences·Stacey S Willard, Shahriar Koochekpour
Feb 24, 2015·Neuropharmacology·Hannah A HathawayJarda T Wroblewski
Mar 15, 2012·Pflügers Archiv : European journal of physiology·Luis CarreteroPedro Domínguez
Jun 28, 2005·Journal of Pineal Research·Tarja KokkolaJarmo T Laitinen
Jun 15, 2006·Molecular Pharmacology·Clotilde Mannoury la CourLaurence Lanfumey
Mar 7, 2008·The Journal of Pharmacology and Experimental Therapeutics·Hasib Salah-UddinR A John Challiss
Dec 30, 2008·Pharmacological Reviews·Francesco FerragutiFerdinando Nicoletti

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