Cell type-specific expression profile and signaling requirements in early hematopoietic reprogramming

Stem Cells and Development
Lan KangQian-Fei Wang

Abstract

Hematopoietic cells represent an attractive starting cell type for induced pluripotent stem (iPS) cells induction, yet the molecular mechanisms in hematopoietic reprogramming are poorly defined. In this study, we showed that long-term hematopoietic stem cells are more amenable for iPS cells induction among several hematopoietic stem and progenitor cell (HSPC) populations, and that this is accompanied by an earlier induction of the transcriptional program that is involved in the promotion of macromolecule metabolism and cell proliferation. Notably, we identified multiple signaling pathways that exhibited distinct expression patterns in HSPCs compared to that of fibroblasts, which is the most commonly used model for probing the somatic reprogramming process. We further experimentally confirmed the differential requirements of the Wnt/β-catenin and transforming growth factor-beta (TGF-β) signaling pathways in these two cell types. These data demonstrate that hematopoietic cells have a cell-type specific transcriptional program and possess unique signaling requirements in the early phase of reprogramming.

References

Mar 29, 2008·Cell Stem Cell·Louise E Purton, David T Scadden
Aug 7, 2008·Cell Stem Cell·Alexander MarsonRudolf Jaenisch
Aug 12, 2008·Cell·In-Hyun ParkGeorge Q Daley
Mar 18, 2009·Bioinformatics·Cole TrapnellSteven L Salzberg
Mar 21, 2009·Blood·Yuin-Han LohGeorge Q Daley
Sep 22, 2009·Current Biology : CB·Nimet Maherali, Konrad Hochedlinger
Mar 18, 2010·Nature Methods·Nils GehlenborgAnne-Claude Gavin
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May 18, 2013·Nature Reviews. Genetics·Yosef BuganimRudolf Jaenisch

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Datasets Mentioned

BETA
GSE54462

Methods Mentioned

BETA
RNA-seq
FACS
PCR

Software Mentioned

Cufflinks
Ensemble
R
TopHat
Ingenuity Pathway Analysis ( IPA )
DEGseq

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