Cell-type specific oxytocin gene expression from AAV delivered promoter deletion constructs into the rat supraoptic nucleus in vivo.

PloS One
Raymond FieldsHarold Gainer

Abstract

The magnocellular neurons (MCNs) in the hypothalamus selectively express either oxytocin (OXT) or vasopressin (AVP) neuropeptide genes, a property that defines their phenotypes. Here we examine the molecular basis of this selectivity in the OXT MCNs by stereotaxic microinjections of adeno-associated virus (AAV) vectors that contain various OXT gene promoter deletion constructs using EGFP as the reporter into the rat supraoptic nucleus (SON). Two weeks following injection of the AAVs, immunohistochemical assays of EGFP expression from these constructs were done to determine whether the EGFP reporter co-localizes with either the OXT- or AVP-immunoreactivity in the MCNs. The results show that the key elements in the OT gene promoter that regulate the cell-type specific expression the SON are located -216 to -100 bp upstream of the transcription start site. We hypothesize that within this 116 bp domain a repressor exists that inhibits expression specifically in AVP MCNs, thereby leading to the cell-type specific expression of the OXT gene only in the OXT MCNs.

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Citations

Nov 11, 2015·Neuroscience Bulletin·Xi Zha, Xiaohong Xu
Apr 29, 2015·Journal of Neuroendocrinology·Gül Dölen
Jan 23, 2015·Science Translational Medicine·Olga PeñagarikanoDaniel H Geschwind
Apr 8, 2016·Journal of Neurophysiology·Yasmine El-ShamaylehGregory D Horwitz
Jul 18, 2012·Nature·Deniz AtasoyScott M Sternson
Jun 14, 2018·Physiological Reviews·Benjamin Jurek, Inga D Neumann

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Methods Mentioned

BETA
transgenic
PCR
transfection

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