PMID: 15381855Sep 24, 2004Paper

Cell volume changes affect gluconeogenesis in the perfused liver of the catfish Clarias batrachus

Journal of Biosciences
Carina GoswamiNirmalendu Saha

Abstract

In addition to lactate and pyruvate, some amino acids were found to serve as potential gluconeogenic substrates in the perfused liver of Clarias batrachus. Glutamate was found to be the most effective substrate, followed by lactate, pyruvate, serine, ornithine, proline, glutamine, glycine, and aspartate. Four gluconeogenic enzymes, namely phosphoenolpyruvate carboxykinase (PEPCK), pyruvate carboxylase (PC), fructose 1,6-bisphosphatase (FBPase) and glucose 6-phosphatase (G6Pase) could be detected mainly in liver and kidney, suggesting that the latter are the two major organs responsible for gluconeogenic activity in this fish. Hypo-osmotically induced cell swelling caused a significant decrease of gluconeogenic efflux accompanied with significant decrease of activities of PEPCK, FBPase and G6Pase enzymes in the perfused liver. Opposing effects were seen in response to hyperosmotically induced cell shrinkage. These changes were partly blocked in the presence of cycloheximide, suggesting that the aniso-osmotic regulations of gluconeogenesis possibly occurs through an inverse regulation of enzyme proteins and/or a regulatory protein synthesis in this catfish. In conclusion, gluconeogenesis appears to play a vital role in C. batrach...Continue Reading

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Citations

Apr 27, 2007·Intensive Care Medicine·Gerd AlbusziesEberhard Barth
Nov 7, 2008·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Kuheli BiswasNirmalendu Saha
Feb 13, 2020·Journal of Experimental Zoology. Part A, Ecological and Integrative Physiology·Tyler G Evans, Dietmar Kültz
Oct 13, 2010·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Caterina FaggioFrancesca Trischitta

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