PMID: 8967439May 1, 1996Paper

Cell volume in vascular smooth muscle is regulated by bumetanide-sensitive ion transport

The American Journal of Physiology
Sergei N OrlovPavel Hamet

Abstract

Vascular smooth muscle cells (VSMC) exhibit shrinkage-induced bumetanide-inhibited 86Rb influx and ethylisopropylamiloride (EIPA)-inhibited 22Na influx. In this study, we examined the role of these transport pathways in volume adjustment of VSMC after isosmotic and hyperosmotic shrinkage. Cell volume was assessed by measurement of [14C]urea distribution. An initial 18-20% cell volume decrease in isosmotically shrunken VSMC was followed by a regulatory volume increase (RVI). RVI was completely abolished by bumetanide but not by EIPA. No RVI was noted in hyperosmotically shrunken VSMC. The initial rate of bumetanide-inhibited 86Rb influx was two- to threefold higher in isosmotically shrunken VSMC than with hyperosmotic shrinkage. Hyperosmotic shrinkage of VSMC was accompanied by a three- to fourfold increase in the rate of bumetanide-inhibited 86Rb efflux, whereas isosmotic shrinkage augmented this component by only 20-30%. In contrast to bumetanide-inhibited 86Rb influx, isosmotic shrinkage slightly increased the rate of EIPA-sensitive 22Na influx. Hyperosmotic shrinkage led to transient activation of EIPA-inhibited 22Na influx, which was completely abolished in 15 min. Activation of adenosine 3',5'-cyclic monophosphate (cAMP) s...Continue Reading

Citations

May 18, 1999·The American Journal of Physiology·W C O'Neill
Jan 5, 2000·Physiological Reviews·J M Russell
Nov 16, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Aidar R GosmanovDonald B Thomason
Jan 23, 2008·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Svetlana V KoltsovaSergei N Orlov
Aug 11, 2010·Hypertension·Zaiming LuoChristopher S Wilcox
Jun 1, 2006·Acta Physiologica·R Franchi-GazzolaO Bussolati
May 13, 2011·American Journal of Physiology. Cell Physiology·Svetlana V KoltsovaSergei N Orlov
Mar 17, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Gengru JiangW Charles O'Neill
Feb 4, 2012·Canadian Journal of Physiology and Pharmacology·Svetlana V KoltsovaSergei N Orlov
Feb 7, 2016·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jennifer LaNickolai O Dulin
Aug 5, 2004·Pflügers Archiv : European journal of physiology·Yana J AnfinogenovaSergei N Orlov
Mar 7, 2003·American Journal of Physiology. Renal Physiology·Alison M DevlinJohanne Tremblay
Mar 10, 1999·The American Journal of Physiology·S N OrlovP Hamet
Nov 27, 2001·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Alexander A. Mongin, Sergei N. Orlov
Jul 3, 2021·International Journal of Molecular Sciences·Parveen BazardBo Ding

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