Cell volume measured by total internal reflection microfluorimetry: application to water and solute transport in cells transfected with water channel homologs

Biophysical Journal
J FarinasA S Verkman

Abstract

Total internal reflection (TIR) microfluorimetry was established as a method to measure continuously the volume of adherent cells and applied to measure membrane permeabilities in cells transfected with water channel homologs. Cytosol was labeled with the membrane-impermeant fluorophore calcein. Fluorescence was excited by the TIR evanescent field in a thin section of cytosol (approximately 150 nm) adjacent to the cell-substrate interface. Because cytosolic fluorophore number per cell remains constant, the TIR fluorescence signal should be inversely related to cell volume. For small volume changes in Sf-9 and LLC-PK1 cells, relative TIR fluorescence was nearly equal to inverse relative cell volume; deviations from the ideal were modeled theoretically. To measure plasma membrane osmotic water permeability, Pf, the time course of osmotically induced cell volume change was inferred from the TIR fluorescence signal. LLC-PK1 cells expressing the CHIP28 water channel had an HgCl2-sensitive, threefold increase in Pf compared to nontransfected cells (Pf = 0.0043 cm/s at 10 degrees C). Solute permeability was measured from the TIR fluorescence time course in response to solute gradients. Glycerol permeability in Sf-9 cells expressing th...Continue Reading

Citations

Jun 5, 2003·European Biophysics Journal : EBJ·E I SolenovL N Ivanova
Jul 1, 2008·Journal of Mathematical Biology·William F Pickard
Jul 27, 2007·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Carissa M Krane, David L Goldstein
Apr 5, 2008·Pflügers Archiv : European journal of physiology·Grazia TammaGiovanna Valenti
Dec 1, 2007·Journal of Biological Physics·Mario ParisiRoxana Toriano
Feb 1, 1997·Current Opinion in Biotechnology·N L Thompson, B C Lagerholm
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·T KatsuraD Brown
Apr 23, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·M OheimW Stühmer
Sep 1, 1997·The Journal of Clinical Investigation·E P CarterA S Verkman
May 29, 1998·The Journal of Clinical Investigation·B K Tamarappoo, A S Verkman
Aug 26, 1998·The Journal of Clinical Investigation·A FrigeriM Svelto
May 17, 2000·The Journal of Clinical Investigation·H MatsuiR C Boucher
Mar 1, 2012·PloS One·Chin Wee TanAntony W Burgess
Mar 15, 2014·Nature Reviews. Drug Discovery·Alan S VerkmanMarios C Papadopoulos
Jul 14, 1997·The Journal of Cell Biology·O SeksekA S Verkman
Apr 30, 2005·Biology of the Cell·Paula FordClaudia Capurro
Dec 20, 2015·Chemical Biology & Drug Design·Rajkumar V PatilAlok K Mitra
Aug 19, 2015·Pharmacology & Therapeutics·Eric BeitzJulia von Bülow
Apr 3, 2007·Anais Da Academia Brasileira De Ciências·Márcio S Rocha, Oscar N Mesquita
Jun 18, 2017·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Michael A Model
Apr 11, 2017·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Casey L BrownJinseok Heo
Dec 20, 2003·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·A D LúcioO N Mesquita
Jul 18, 2018·Faraday Discussions·Andreas Horner, Peter Pohl
Jan 1, 2008·Journal of Biomedical Optics·Manuel KemmlerChristian Hoffmann
Apr 3, 2001·Nature Reviews. Molecular Cell Biology·J A Steyer, W Almers
Jan 18, 2006·Journal of Biomedical Optics·Miriam S DrosteRoger Wepf
Nov 6, 2009·American Journal of Physiology. Renal Physiology·R A FentonM Rützler
Sep 12, 1998·The Journal of Biological Chemistry·H TsukaguchiM A Hediger
Jul 12, 2002·American Journal of Physiology. Renal Physiology·Marina ZeleninaAnita Aperia
Nov 22, 2018·Applied Optics·Abdollah HassanzadehSalah Raza Saeed
Feb 8, 2000·American Journal of Physiology. Renal Physiology·A S Verkman, A K Mitra
Jan 1, 1996·The American Journal of Physiology·A S VerkmanJ Farinas
Jun 5, 1999·Methods : a Companion to Methods in Enzymology·J R Inglefield, R D Schwartz-Bloom
Nov 20, 1998·The American Journal of the Medical Sciences·A S Verkman
Oct 1, 1995·Kidney International·A S VerkmanT Ma

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