Cell volume regulates liver phosphoenolpyruvate carboxykinase and fructose-1,6-bisphosphatase genes

The American Journal of Physiology
S Kaiser

Abstract

Hypertonic-induced cell shrinkage increases glucose release in H-4-II-E rat hepatoma cells. This is paralleled by a concomitant increase in the mRNA levels of the rate-limiting enzymes of the pathway of gluconeogenesis, phosphoenolpyruvate carboxykinase (PCK) and fructose-1,6-bisphosphatase (FBP), of seven- and fivefold, respectively. In contrast, hypotonic-induced swelling of the cells results in a transient decrease in PCK and FBP mRNAs to 15% and 39% of control levels. The antagonistic effects of hyper- and hypotonicity mimic the counteracting effects of adenosine 3',5'-cyclic monophosphate (cAMP) and insulin on PCK and FBP mRNA levels. The hypertonic-induced increase in mRNA levels is due to an enhanced transcriptional rate, whereas the decrease in mRNAs caused by hypotonicity results from a decrease in transcription as well as mRNA stability. The inductive effect of hypertonicity does not require ongoing protein synthesis and acts independently of the cAMP-dependent protein kinase and protein kinase C pathways. These results suggest that cell volume changes in liver cells may play an important role in regulating hepatic glucose metabolism by altered gene expression.

References

Oct 1, 1992·Physiological Reviews·N A McCarty, R G O'Neil
Mar 15, 1992·The Biochemical Journal·M al-HaboriL Agius
Jun 1, 1992·The Journal of Clinical Investigation·A PietrangeloD A Shafritz
Dec 12, 1991·Biochimica Et Biophysica Acta·D Häussinger, F Lang
Aug 1, 1989·The American Journal of Physiology·M E Chamberlin, K Strange
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M R el-MaghrabiS J Pilkis
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·H Yoo-WarrenR W Hanson
Mar 19, 1993·Science·J L BrewsterM C Gustin

❮ Previous
Next ❯

Citations

Jan 21, 2016·The Journal of Pharmacology and Experimental Therapeutics·Jennifer L HarmonRick G Schnellmann

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adrenocortical Carcinoma

Hepatocellular carcinoma is the most common type of primary liver cancer and frequently occurs in individuals with chronic liver diseases like cirrhosis. Here is the latest research.

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.