PMID: 9547321Apr 21, 1998Paper

Cell volume regulation in proximal renal tubules from trout (Salmo trutta)

The Journal of Experimental Biology
H Kanli, E Norderhus

Abstract

Regulatory volume decrease (RVD) following hypo-osmotic stimulation was studied using videometric methods in isolated proximal renal tubules from trout (Salmo trutta). The relative tubule diameter increased by 132.0+/-4.8 % (maximum swelling within 1 min at 15 and 25 degrees C and within 4 min at 10 degrees C) following a change from iso-osmotic (290 mosmol kg-1) to hypo-osmotic (160 mosmol kg-1) Ringer's solution. The tubule diameter subsequently decreased to approximately one-quarter of the maximal value. Ouabain (1 mmol l-1) reduced cell swelling and inhibited the RVD response by 28.0+/-10.5 %. Furthermore, increasing the bath K+ concentration by 30 mmol l-1 inhibited RVD by 76.5+/-3.6 %. The K+ channel blocker quinine, but not Ba2+ (1 and 2 mmol l-1), significantly decreased the RVD response (by 25.0+/-5.4 and 72.3+/-5.1 % at 0.1 and 0.5 mmol l-1, respectively). Similarly, increasing the Cl- concentration in the bath from 47 to 102 mmol l-1 induced a significant reduction (45. 2+/-7.9 %) in RVD. The RVD response was also markedly reduced (by 54. 7+/-5.3 %) by the Cl- channel blocker indacrinone (MK-196; 0.5 mmol l-1), but only marginally by 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB; 1, 5, 8 and 10 micromol l-1). Add...Continue Reading

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