Cell wall 1,6-beta-glucan synthesis in Saccharomyces cerevisiae depends on ER glucosidases I and II, and the molecular chaperone BiP/Kar2p

The EMBO Journal
J F SimonsA Helenius

Abstract

The role of glucose trimming in the endoplasmic reticulum of Saccharomyces cerevisiae was investigated using glucosidase inhibitors and mutant strains devoid of glucosidases I and II. These glucosidases are responsible for removing glucose residues from the N-linked core oligosaccharides attached to newly synthesized polypeptide chains. In mammalian cells they participate together with calnexin, calreticulin and UDP-glucose:glycoprotein glucosyltransferase in the folding and quality control of newly synthesized glycoproteins. In S.cerevisiae, glucosidase II is encoded by the GLS2 gene, and glucosidase I, as suggested here, by the CWH41 gene. Using castanospermine (an alpha-glucosidase inhibitor) and yeast strains defective in glucosidase I, glucosidase II and BiP/Kar2p, it was demonstrated that cell wall synthesis depends on the two glucosidases and BiP/Kar2p. In double mutants with defects in both BiP/Kar2p and either of the glucosidases the phenotype was particularly clear: synthesis of 1,6-beta-glucan_a cell wall component_was reduced; the cell wall displayed abnormal morphology; the cells aggregated; and their growth was severely inhibited. No defects in protein folding or secretion could be detected. We concluded that gluc...Continue Reading

References

Oct 24, 1977·Archives of Microbiology·M Horisberger, M Vonlanthen
Jan 2, 1992·Nature·M J Gething, J Sambrook
Oct 24, 1991·Nature·G C FlynnJ E Rothman
May 1, 1990·The Journal of Cell Biology·C BooneH Bussey
Jun 1, 1990·The Journal of Cell Biology·J P VogelM D Rose
Jan 1, 1985·Annual Review of Biochemistry·R Kornfeld, S Kornfeld
Jun 1, 1969·Archives of Biochemistry and Biophysics·J F McKelvy, Y C Lee
Nov 24, 1983·Nature·I G Haas, M Wabl
Jul 15, 1995·European Journal of Biochemistry·B Kalz-FüllerE Bause
Jul 1, 1995·The Journal of Cell Biology·J F SimonsA Helenius
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·C HammondA Helenius

❮ Previous
Next ❯

Citations

Apr 2, 2010·Antonie van Leeuwenhoek·María D Frade-PérezHéctor M Mora-Montes
Apr 5, 2005·Methods : a Companion to Methods in Enzymology·E Sergio Trombetta, Armando J Parodi
Nov 26, 2004·Molecular Biology of the Cell·Quan ZhongMiriam L Greenberg
May 26, 1998·The EMBO Journal·M BickleM N Hall
Jun 23, 2009·Glycobiology·Robert P QuinnColin J Stirling
Nov 8, 2005·The Plant Cell·Jan SchirawskiRegine Kahmann
Mar 11, 1999·The Plant Cell·N Leborgne-CastelJ Denecke
Jan 11, 2011·Applied and Environmental Microbiology·Sabine SchmidtStefan Martens
Mar 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·Katherine A BorkovichRobert Pratt
Nov 22, 2007·PloS One·Di WuAlan Michael Tartakoff
Dec 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Manasi ChavanWilliam Lennarz
Nov 13, 1998·The Journal of Cell Biology·A Spang, R Schekman
Mar 20, 2002·The Journal of Cell Biology·C Stewart GillmorChris Somerville
Aug 19, 2009·Proteomics·Rafael Prados-RosalesAntonio Di Pietro
Dec 16, 2006·The EMBO Journal·Catherine E JessopNeil J Bulleid
Aug 31, 2000·Annual Review of Biochemistry·A J Parodi
Sep 17, 1998·The Journal of Biological Chemistry·J SaarelaL Peltonen
Mar 3, 2007·The Biochemical Journal·Seema ChakravarthiNeil J Bulleid

❮ Previous
Next ❯

Related Concepts