Cell Wall Acetylation in Hybrid Aspen Affects Field Performance, Foliar Phenolic Composition and Resistance to Biological Stress Factors in a Construct-Dependent Fashion

Frontiers in Plant Science
Marta Derba-MaceluchEwa J Mellerowicz

Abstract

The production of biofuels and "green" chemicals from the lignocellulose of fast-growing hardwood species is hampered by extensive acetylation of xylan. Different strategies have been implemented to reduce xylan acetylation, resulting in transgenic plants that show good growth in the greenhouse, improved saccharification and fermentation, but the field performance of such plants has not yet been reported. The aim of this study was to evaluate the impact of reduced acetylation on field productivity and identify the best strategies for decreasing acetylation. Growth and biological stress data were evaluated for 18 hybrid aspen lines with 10-20% reductions in the cell wall acetyl content from a five year field experiment in Southern Sweden. The reduction in acetyl content was achieved either by suppressing the process of acetylation in the Golgi by reducing expression of REDUCED WALL ACETYLATION (RWA) genes, or by post-synthetic acetyl removal by fungal acetyl xylan esterases (AXEs) from two different families, CE1 and CE5, targeting them to cell walls. Transgene expression was regulated by either a constitutive promoter (35S) or a wood-specific promoter (WP). For the majority of transgenic lines, growth was either similar to that...Continue Reading

References

Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·Z Xin, J Browse
Sep 22, 2000·Plant Molecular Biology·S M KaepplerY Rhee
Jul 1, 1995·The Plant Cell·R. A. Dixon, N. L. Paiva
Dec 9, 2004·The Plant Journal : for Cell and Molecular Biology·John P VogelShauna C Somerville
Feb 24, 2007·The Plant Journal : for Cell and Molecular Biology·Zhanguo XinJohn Browse
Feb 24, 2010·Journal of Chemical Ecology·Benjamin A BabstChung-Jui Tsai
Dec 1, 2010·Journal of Chemical Ecology·Ashley N ScioneauxAnn E Hagerman
Mar 1, 2011·Phytochemistry·Raymond V Barbehenn, C Peter Constabel
Mar 17, 2011·PloS One·Valérie LefebvreMylène Durand-Tardif
Jul 13, 2011·Journal of Chemical Ecology·Ilka Nacif AbreuBenedicte Riber Albrectsen
Sep 9, 2011·Cold Spring Harbor Symposia on Quantitative Biology·L Cai, B P Tu
Sep 13, 2011·Plant Molecular Biology·Gennady PogorelkoOlga A Zabotina
May 29, 2012·Frontiers in Plant Science·Sascha Gille, Markus Pauly
Jun 5, 2013·Frontiers in Plant Science·Prashant Mohan-Anupama PawarEwa J Mellerowicz
Jun 7, 2014·Frontiers in Plant Science·Daniela BellincampiVincenzo Lionetti
Aug 22, 2014·The Plant Journal : for Cell and Molecular Biology·Breeanna R UrbanowiczWilliam S York
Oct 10, 2014·PloS One·Ken Keefover-RingBenedicte Riber Albrectsen
May 9, 2015·The New Phytologist·Michael D Madritch, Richard L Lindroth
May 12, 2015·Plant Biotechnology Journal·Prashant Mohan-Anupama PawarEwa J Mellerowicz
Jun 4, 2016·Molecular Cell·Jean-Pierre Etchegaray, Raul Mostoslavsky
Oct 19, 2016·Biotechnology Advances·Peter BielyVladimír Puchart
Mar 4, 2017·The New Phytologist·Prashant Mohan-Anupama PawarEwa J Mellerowicz
Apr 22, 2017·Biotechnology for Biofuels·Prashant Mohan-Anupama PawarEwa J Mellerowicz
Jul 12, 2017·Molecular Cell·Sharanya SivanandKathryn E Wellen
Dec 22, 2017·The Plant Journal : for Cell and Molecular Biology·Laura BaceteAntonio Molina
May 23, 2018·Proceedings of the National Academy of Sciences of the United States of America·Yinon M Bar-OnRon Milo
Sep 7, 2018·Frontiers in Plant Science·Markus Pauly, Vicente Ramírez
Nov 9, 2018·Frontiers in Plant Science·Evgeniy DonevEwa J Mellerowicz
Mar 28, 2019·Tree Physiology·Geraldine Gourlay, C Peter Constabel
Sep 26, 2019·Plant Physiology·Karen J KlothBenedicte R Albrectsen

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Methods Mentioned

BETA
acetylation
transgenic
environmental stress
genetic
genetic modification
transgenics
histone acetylation
genetic modifications

Software Mentioned

QuanLynx
Adobe Photoshop
Axiovision V
JMP
MassLynx

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