Cell wall integrity modulates RHO1 activity via the exchange factor ROM2

The EMBO Journal
M BickleMichael N Hall

Abstract

The essential phosphatidylinositol kinase homologue TOR2 of Saccharomyces cerevisiae controls the actin cytoskeleton by activating a GTPase switch consisting of RHO1 (GTPase), ROM2 (GEF) and SAC7 (GAP). We have identified two mutations, rot1-1 and rot2-1, that suppress the loss of TOR2 and are synthetic-lethal. The wild-type ROT1 and ROT2 genes and a multicopy suppressor, BIG1, were isolated by their ability to rescue the rot1-1 rot2-1 double mutant. ROT2 encodes glucosidase II, and ROT1 and BIG1 encode novel proteins. We present evidence that cell wall defects activate RHO1. First, rot1, rot2, big1, cwh41, gas1 and fks1 mutations all confer cell wall defects and suppress tor2(ts). Second, destabilizing the cell wall by supplementing the growth medium with 0.005% SDS also suppresses a tor2(ts) mutation. Third, disturbing the cell wall with SDS or a rot1, rot2, big1, cwh41, gas1 or fks1 mutation increases GDP/GTP exchange activity toward RHO1. These results suggest that cell wall defects suppress a tor2 mutation by activating RHO1 independently of TOR2, thereby inducing TOR2-independent polarization of the actin cytoskeleton and cell wall synthesis. Activation of RHO1, a subunit of the cell wall synthesis enzyme glucan synthase,...Continue Reading

References

Jul 1, 1991·The Journal of Cell Biology·J A ShawE Cabib
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A Bender, J R Pringle
Feb 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P MadauleA M Myers
Mar 1, 1984·The Journal of Cell Biology·J V Kilmartin, A E Adams
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·C M DouglasM el-Sherbeini
Oct 5, 1995·Nature·E J BrownS L Schreiber
Apr 14, 1995·Science·E A Clark, J S Brugge
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·H QadotaY Ohya
Jul 1, 1994·Yeast·F M Klis
Mar 1, 1994·Trends in Biochemical Sciences·J J BergeronD B Williams
Apr 19, 1996·The Journal of Biological Chemistry·Y KamadaD E Levin
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·S R von ManteuffelG Thomas
Jan 1, 1996·Molecular Biology of the Cell·N C BarbetM N Hall
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·A SchmidtM N Hall
Nov 18, 1997·Glycobiology·P A RomeroH Bussey
Jan 13, 1998·Current Opinion in Cell Biology·G Thomas, M N Hall

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Citations

May 20, 2009·The Journal of Membrane Biology·Nathan C RockwellJeremy Thorner
Nov 24, 2004·Applied Microbiology and Biotechnology·M ReverberiU Tomati
Mar 6, 1999·Current Opinion in Microbiology·N H Georgopapadakou
May 16, 2013·Journal of Natural Products·Dayna EmrickLeif Smith
Jan 31, 2009·Nature Reviews. Drug Discovery·Jia JiaYu Zong Chen
Sep 5, 2002·Molecular Microbiology·Anja SchmidtMichael N Hall
Feb 26, 2000·Molecular Biology of the Cell·B Santos, M Snyder
Nov 1, 2003·Molecular Biology of the Cell·Amy K A deHartLinda Hicke
May 30, 2008·Molecular Biology of the Cell·Masato TakeuchiKenji Kohno
Feb 16, 2002·The EMBO Journal·M Cristina CruzJoseph Heitman
Dec 16, 2005·Molecular Plant-microbe Interactions : MPMI·Zaira CaracuelM Isabel G Roncero
Nov 8, 2005·The Plant Cell·Jan SchirawskiRegine Kahmann
Feb 3, 2012·Science Signaling·Caleb M CardonJared Rutter
Jun 24, 2009·Molecular and Cellular Biology·Miriam SchonbrunRonit Weisman
Jun 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Guillaume Lesage, Howard Bussey
Jun 1, 2002·Microbiology and Molecular Biology Reviews : MMBR·Stefan Hohmann
Jun 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·David E Levin
Jan 19, 1999·Annual Review of Cell and Developmental Biology·A Schmidt, M N Hall
Nov 3, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Katsuya KajimotoJunichi Sadoshima
Sep 27, 2007·PloS One·Dietmar Dirnberger, Klaus Seuwen
Dec 17, 2011·Genetics·Robbie Loewith, Michael N Hall
Nov 13, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jing HuangStuart L Schreiber

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