Cell-wall invertases from rice are differentially expressed in Caryopsis during the grain filling stage

Journal of Integrative Plant Biology
Yong-Qin WangZhen Zhu

Abstract

Cell-wall invertase plays an important role in sucrose partitioning between source and sink organs in higher plants. To investigate the role of cell-wall invertases for seed development in rice (Oryza sativa L.), cDNAs of three putative cell-wall invertase genes OsCIN1, OsCIN2 and OsCIN3 were isolated. Semi-quantitative reverse transcription-polymerase chain reaction analysis revealed different expression patterns of the three genes in various rice tissues/organs. In developing caryopses, they exhibited similar temporal expression patterns, expressed highly at the early and middle grain filling stages and gradually declined to low levels afterward. However, the spatial expression patterns of them were very different, with OsCIN1 primarily expressed in the caryopsis coat, OsCIN2 in embryo and endosperm, and OsCIN3 in embryo. Further RNA in situ hybridization analysis revealed that a strong signal of OsCIN2 mRNA was detected in the vascular parenchyma surrounding the xylem of the chalazal vein and the aleurone layer, whereas OsCIN3 transcript was strongly detected in the vascular parenchyma surrounding the phloem of the chalazal vein, cross-cells, the aleurone layer and the nucellar tissue. These data indicate that the three cell...Continue Reading

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Citations

Jun 19, 2018·Journal of Obstetrics and Gynaecology : the Journal of the Institute of Obstetrics and Gynaecology·Aslı Yarcı GursoyGamze Sinem Caglar
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Oct 17, 2015·Journal of Integrative Plant Biology·Xiaoba WuChun-Ming Liu
Nov 3, 2020·AoB Plants·Sintayehu D DabaMohsen Mohammadi
Dec 29, 2020·Journal of Experimental Botany·Carla Coluccio LeskowFernando Carrari
Feb 3, 2018·Journal of Agricultural and Food Chemistry·Xiaotong GuoBei Li

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