PMID: 780112Jul 1, 1976

Cell-wall lipopolysaccharides of ampicillin-resistant mutants of Escherichia coli K-12

European Journal of Biochemistry
P PrehmH G Boman

Abstract

The lipopolysaccharides of ampicillin-resistant cell-wall-defective mutants of Escherichia coli K-12 were analyzed. From their lipopolysaccharides the respective core oligosaccharides were obtained. Following dephosphorylation,the core oligosaccharides were methylated and analyzed by gas chromatography/mass spectrometry. From core-defective mutants substructures of the K-12 core were obtained. Analysis of the lipopolysaccharide preparations from wild-type K-12 indicated the presence of several core structures with different degrees of completion. The lipopolysaccharide preparation was degraded and the oligosaccharide mixture was partially resolved by gel filtration chromatography. Methylation, gas chromatography and mass spectrometry of the oligosaccharides permitted the tentative formulation of the K-12 core structure. Alternative interpretations for this heterogeneity are discussed.

References

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Citations

Feb 1, 1975·Journal of Bacteriology·H G Boman, D A Monner
Aug 1, 1975·European Journal of Biochemistry·P PrehmK Jann
Jun 11, 1971·Annals of the New York Academy of Sciences·H G BomanG D Bloom
Dec 4, 1972·European Journal of Biochemistry·K Reske, K Jann
Jul 1, 1973·Journal of General Microbiology·G Schmidt
Jul 1, 1967·European Journal of Biochemistry·B Jann, K Jann
Dec 16, 1965·Biochimica Et Biophysica Acta·H NikaidoA M Rapin
Jun 30, 1966·Annals of the New York Academy of Sciences·A M Rapin, H Mayer
Jun 1, 1969·European Journal of Biochemistry·C GalanosO Westphal
May 4, 1962·Biochemical and Biophysical Research Communications·T OKAZAKIS SUZUKI

Related Concepts

Rhamnose, L-Isomer
Alkalescens-Dispar Group
Protein Methylation
Penicillin Resistance
Methylate
Glucose, (beta-D)-Isomer
Galactose
Polysaccharides, Bacterial
Mass Spectrometry
Gel Chromatography

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