Cell wall modification in grapevine cells in response to UV stress investigated by atomic force microscopy

Ultramicroscopy
E LesniewskaA Pugin

Abstract

Despite cell wall reinforcement being a well-known defence mechanism of plants, it remains poorly characterized from a physical point of view. The objective of this work was to further describe this mechanism. Vitis vinifera cv Gamay cells were treated with UV-light (254 nm), a well-known elicitor of defence mechanisms in grapevines, and physical cell wall modifications were observed using the atomic force microscopy (AFM) under native conditions. The grapevine cell suspensions were continuously observed in their culture medium from 30 min to 24h after elicitation. In the beginning, cellulose fibrils covered by a matrix surrounded the control and treated cells. After 3 h, the elicited cells displayed sprouted expansions around the cell wall that correspond to pectin chains. These expansions were not observed on untreated grapevine cells. The AFM tip was used to determine the average surface elastic modulus of cell wall that account for cell wall mechanical properties. The elasticity is diminished in UV-treated cells. In a comparative study, grapevine cells showed the same decrease in cell wall elasticity when treated with a fungal biotic elicitor of defence response. These results demonstrate cell wall strengthening by UV stress.

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Citations

Jul 23, 2013·Journal of Experimental Botany·Pascale MilaniArezki Boudaoud
Jul 8, 2010·Plant Cell Reports·Glória Maria de Farias Viégas AquijeAntonio Alberto Ribeiro Fernandes
Jan 1, 2015·Plants·Hyacinthe Le GallCatherine Rayon
Apr 20, 2016·International Journal of Molecular Sciences·Anuar Magaña-ÁlvarezAntonio Alberto Ribeiro Fernandes
Aug 24, 2017·Seminars in Cell & Developmental Biology·S KasasG Dietler
Feb 23, 2021·Plant Physiology and Biochemistry : PPB·Aleksandra Lj MitrovićKsenija Radotić

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