PMID: 11903965Mar 21, 2002Paper

Cell wall modifications of bean (Phaseolus vulgaris) cell suspensions during habituation and dehabituation to dichlobenil

Physiologia Plantarum
Antonio EncinaJesús M Alvarez

Abstract

Bean (Phaseolus vulgaris L.) cell suspensions were adapted for growth in 12 &mgr;M dichlobenil (2,6-dichlorobenzonitrile or DCB) by a stepwise increase in the concentration of the inhibitor in each subculture. Non-tolerant suspensions (I50 = 0.3 &mgr;M) gave rise to single cells or small clusters while tolerant cell suspensions (I50 = 30 &mgr;M) grown in DCB formed large clusters. The cells in these clusters were surrounded by a thick and irregular cell wall with a lamellate structure and lacking a differentiated middle lamella. Analysis of habituated cell walls by Fourier transform infrared spectroscopy and cell wall fractionation revealed: (1) a reduced amount of cellulose and hemicelluloses, mainly xyloglucan (2) qualitative and quantitative differences in pectin levels, and (3) a non-crystalline and soluble beta-1,4-glucan. When tolerant cells were returned to medium lacking DCB, the size of the cell clusters was reduced; the middle lamella was only partly formed, and the composition of the cell wall gradually reverted to that obtained with non-tolerant cells. However, dehabituated cells (I50 = 12 &mgr;M) were 40-fold more tolerant to DCB than non-tolerant cells and were only 2.5-fold more sensitive than tolerant cells.

References

Aug 1, 1973·Analytical Biochemistry·N Blumenkrantz, G Asboe-Hansen
Jun 1, 1973·Analytical Biochemistry·C Schwabe
Dec 1, 1969·Analytical Biochemistry·D M Updegraff
Feb 21, 1998·Science·T ArioliR E Williamson
May 16, 2000·The Plant Cell·R A BurtonG B Fincher
Feb 13, 2001·Plant Science : an International Journal of Experimental Plant Biology·A E. EncinaJ M. Álvarez
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Deborah P. Delmer

❮ Previous
Next ❯

Citations

Dec 18, 2013·Journal of Plant Physiology·María de CastroJosé Luis Acebes
Feb 3, 2009·Plant & Cell Physiology·Dyani LewisEdward J Newbigin
Apr 7, 2009·Journal of Plant Physiology·Penélope García-AnguloJesús M Álvarez
Sep 28, 2004·The Plant Journal : for Cell and Molecular Biology·Iain W ManfieldWilliam G T Willats
Mar 5, 2015·Journal of Integrative Plant Biology·Hugo MélidaAntonio Encina
May 31, 2015·Plant Science : an International Journal of Experimental Plant Biology·Silvia FornaléDavid Caparros-Ruiz
Oct 16, 2009·Plant Signaling & Behavior·Penélope García-AnguloJosé L Acebes
Jun 20, 2016·Plant Physiology and Biochemistry : PPB·Asier Largo-GosensJesús M Álvarez
Apr 11, 2012·International Journal of Molecular Sciences·Penélope García-AnguloJosé L Acebes
Jan 14, 2018·Planta·J Mabel Hernández-AltamiranoPenélope García-Angulo
Feb 22, 2018·Physiologia Plantarum·Romina Martínez-RubioAnna Kärkönen
Dec 21, 2006·Protoplasma·D Volkmann, F Baluska
Jan 16, 2007·Plant Physiology·Maureen C McCannNicholas C Carpita
Aug 29, 2021·Plants·Alfonso Gonzalo De la RubiaPenélope García-Angulo

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.