Cell wall peroxidases in the liverwort Dumortiera hirsuta are responsible for extracellular superoxide production, and can display tyrosinase activity

Physiologia Plantarum
Jackson L Y LiF V Minibayeva

Abstract

In our earlier work, we showed that the liverwort Dumortiera hirsuta produces an extracellular oxidative burst of superoxide radicals during rehydration following desiccation stress. The oxidative burst is a common early response of organisms to biotic and abiotic stresses, with suggested roles in signal transduction, formation of protective substances such as suberin, melanin and lignin and defense against pathogens. To discover which enzymes are responsible for the extracellular superoxide production, we isolated apoplastic fractions from D. hirsuta, surveyed for the presence of potential redox enzymes, and performed non-denaturing polyacrylamide gel electrophoresis activity stains. Various isoforms of peroxidase (EC 1.11.1.7) and tyrosinase (o-diphenolase) (EC 1.10.3.1) were present at significant levels in the apoplast. In-gel activity staining revealed that some peroxidases isoforms could produce superoxide, while tryosinases could readily metabolize 3,4-dihydroxy phenyl l-alanine (l-dopa) into melanins. Interestingly, some peroxidase isoforms could oxidize the native tyrosinase substrate l-dopa at significant levels, even in the absence of hydrogen peroxide, while others could do so only in the presence of hydrogen peroxi...Continue Reading

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Citations

Jan 8, 2013·Doklady Biological Sciences : Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections·A V ChasovF V Minibayeva
Mar 13, 2013·Doklady. Biochemistry and Biophysics·E V PradedovaR K Salyaev
May 4, 2012·Plant Physiology and Biochemistry : PPB·Igor CesarinoPaulo Mazzafera
Sep 22, 2010·The New Phytologist·Ilse KrannerCharlotte E Seal
Nov 12, 2015·Biochemistry. Biokhimii︠a︡·A V ChasovF V Minibayeva
Jul 19, 2016·Phytochemistry·Mónika VargaAndrás Palágyi
Aug 23, 2019·The New Phytologist·Andrea BuschSabine Zachgo
Apr 1, 2017·Frontiers in Plant Science·Inés Ponce de León, Marcos Montesano
Sep 8, 2017·Frontiers in Plant Science·Anna PodgórskaBożena Szal

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