PMID: 13880442Mar 2, 1962

Cell wall replication in Streptococcus pyogenes

Science
R M COLE, J J HAHN

Abstract

Group A streptococci (Streptococcus pyogenes) of several different serological types were grown in fluorescein-labeled homologous type or group-specific globulins, thereby labeling the antigen-containing cell walls. Specific precipitation or inhibition of the labeled antibody, followed by continued incubation and examination at intervals by ultraviolet, phase, and dark-field microscopy, showed that new cell wall was then nonfluorescent. These nonfluorescent portions were differentiated by a reverse technique of culture in unlabeled globulin, followed by antibody precipitation, further growth, and fluorescent-antibody staining. This technique of differential labeling of cell wall has permitted following, for the first time in a living system, the fate of cell wall formed at different times. The results suggest that cell wall synthesis in actively growing cultures usually occurs simultaneously at at least two sites per coccus, each site representing stages in successive divisions, and that cell wall growth in Streptococcus pyogenes is not by diffuse intercalation with old wall, but is initiated at and extends both peripherally and centripetally from the coccal equator.

Citations

Oct 1, 1963·Journal of Cellular Physiology·P L KUEMPEL, A B PARDEE
Jan 1, 1981·Zeitschrift für allgemeine Mikrobiologie·S Kretschmer
Jan 1, 1967·Zeitschrift für Zellforschung und mikroskopische Anatomie·T N TahmisianB J Wright
Jan 1, 1968·Archiv für Mikrobiologie·R Marchant, D G Smith
Jan 1, 1988·Archives of Microbiology·K Gruber, U B Sleytr
Jan 1, 1964·Folia Microbiologica·J CHALOUPKAP KRECKOVA
Apr 28, 1970·Journal of Molecular Biology·D C Morrison, H J Morowitz
Oct 1, 1993·Current Opinion in Biotechnology·Vincent A FischettiG Pozzi
Dec 1, 1996·Current Opinion in Biotechnology·Vincent A FischettiG Pozzi
Oct 16, 2002·Trends in Microbiology·Arthur L Koch
Apr 23, 2003·Trends in Microbiology·Arthur L Koch
Jul 14, 2005·Nature Reviews. Microbiology·Matthew T Cabeen, Christine Jacobs-Wagner
Nov 20, 2008·Proceedings of the National Academy of Sciences of the United States of America·Assaf Raz, Vincent A Fischetti
Jan 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·A N CHATTERJEE, J T PARK
Dec 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·H E KUBITSCHEK
Aug 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·E W Green, M Schaechter
Dec 27, 2011·Molecular & Cellular Proteomics : MCP·Cesira L GaleottiRenata Grifantini
Mar 1, 1966·The Journal of Cell Biology·J M Schmidt, R Y Stanier
Apr 1, 1963·The Journal of Experimental Medicine·J J HAHN, R M COLE
Nov 1, 1963·The Journal of Experimental Medicine·J J HAHN, R M COLE
Nov 1, 1969·The Journal of Experimental Medicine·J SwansonE C Gotschlich
Feb 26, 2010·Cold Spring Harbor Perspectives in Biology·Esteban Toro, Lucy Shapiro
Jun 1, 1976·Antimicrobial Agents and Chemotherapy·V Lorian, B Atkinson
Aug 31, 2000·Applied and Environmental Microbiology·Arthur L Koch
Oct 15, 2003·Clinical Microbiology Reviews·Arthur L Koch
Apr 10, 2007·Journal of Bacteriology·Andrea C DeDentOlaf Schneewind
Nov 14, 2006·Journal of Bacteriology·Daniela FaddaOrietta Massidda
Dec 13, 2005·Microbiology and Molecular Biology Reviews : MMBR·Dirk-Jan Scheffers, Mariana G Pinho
Feb 1, 1964·Canadian Journal of Microbiology·K L ChungP K Isaac
Jun 7, 2006·Annual Review of Microbiology·June R Scott, Timothy C Barnett
Jan 31, 2009·PLoS Pathogens·Fredric CarlssonEric J Brown
Nov 29, 2013·Proceedings of the National Academy of Sciences of the United States of America·Michael S GilmoreDaria Van Tyne
May 1, 1971·CRC Critical Reviews in Microbiology·M L Higgins, G D Shockman
May 10, 1974·Annals of the New York Academy of Sciences·G D ShockmanM L Higgins
Jun 21, 1971·Annals of the New York Academy of Sciences·J FranĕkO Procházka
Jan 22, 2005·Molecular Microbiology·Beatriz LaraOrietta Massidda
Mar 28, 2008·Molecular Microbiology·Dennis ClaessenDavid H Edwards
Apr 20, 2012·Molecular Microbiology·Assaf RazVincent A Fischetti
Oct 4, 2008·Microbiology·Antoni P A HendrickxRob J L Willems
Feb 13, 2008·FEMS Microbiology Reviews·André ZapunMariana G Pinho
Dec 17, 1971·Nature·A Tomasz
Jan 1, 1976·Journal of Dental Research·G D ShockmanB Terleckyj
Oct 14, 2017·FEMS Microbiology Reviews·Ditte Høyer EngholmRikke Schmidt Kjærgaard
Aug 25, 2006·Nature·Fredric CarlssonGunnar Lindahl
Feb 19, 2005·The Journal of Biological Chemistry·Luciano A Marraffini, Olaf Schneewind
Feb 15, 1983·European Journal of Biochemistry·M T Davison, P B Garland
Jan 25, 2006·Molecular Microbiology·Susanne M Rafelski, Julie A Theriot
Dec 7, 1965·Archiv für Mikrobiologie·D A Kuhn, M P Starr
Feb 18, 2011·Nature Reviews. Microbiology·Antoni P A HendrickxOlaf Schneewind
Dec 1, 1965·Canadian Journal of Microbiology·K L ChungP K Isaac
Feb 1, 1979·Acta Pathologica Et Microbiologica Scandinavica. Section B, Microbiology·C H BertholdC E Nord
Apr 28, 2000·Microbiology·B MartínezJ E Suárez
Sep 29, 2006·Microbiology·Girbe BuistOscar P Kuipers

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