Cell wall structure suitable for surface display of proteins in Saccharomyces cerevisiae

Yeast
Hiroyuki MatsuokaMasayuki Azuma

Abstract

A display system for adding new protein functions to the cell surfaces of microorganisms has been developed, and applications of the system to various fields have been proposed. With the aim of constructing a cell surface environment suitable for protein display in Saccharomyces cerevisiae, the cell surface structures of cell wall mutants were investigated. Four cell wall mutant strains were selected by analyses using a GFP display system via a GPI anchor. β-Glucosidase and endoglucanase II were displayed on the cell surface in the four mutants, and their activities were evaluated. mnn2 deletion strain exhibited the highest activity for both the enzymes. In particular, endoglucanase II activity using carboxymethylcellulose as a substrate in the mutant strain was 1.9-fold higher than that of the wild-type strain. In addition, the activity of endoglucanase II released from the mnn2 deletion strain by Zymolyase 20T treatment was higher than that from the wild-type strain. The results of green fluorescent protein (GFP) and endoglucanase displays suggest that the amounts of enzyme displayed on the cell surface were increased by the mnn2 deletion. The enzyme activity of the mnn2 deletion strain was compared with that of the wild-type...Continue Reading

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Citations

Jun 27, 2015·Current Opinion in Chemical Biology·Tomohisa HasunumaAkihiko Kondo
Jun 14, 2015·Biotechnology Advances·Tsutomu Tanaka, Akihiko Kondo
Sep 23, 2014·FEMS Yeast Research·Tsutomu Tanaka, Akihiko Kondo
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Oct 12, 2014·Applied and Environmental Microbiology·Jungu BaeMitsuyoshi Ueda
Feb 13, 2018·Applied Microbiology and Biotechnology·Cecilia Andreu, Marcel Lí Del Olmo
Jan 10, 2020·FEMS Yeast Research·Eun Joong Oh, Yong-Su Jin
Feb 10, 2019·Applied Microbiology and Biotechnology·Apisan PhienluphonSurisa Suwannarangsee
Jul 31, 2019·Applied Microbiology and Biotechnology·Jesús Urbar-UlloaJorge Verdín
Jan 4, 2021·Bioprocess and Biosystems Engineering·Mengqi YeGuanjun Chen

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