Cellobiose dehydrogenase, an active agent in cellulose depolymerization.

Applied and Environmental Microbiology
S D MansfieldJohn N Saddler

Abstract

The ability of cellobiose dehydrogenase purified from Phanerochaete chrysosporium to modify a Douglas fir kraft pulp was assessed. Although the addition of cellobiose dehydrogenase alone had little effect, supplementation with cellobiose and iron resulted in a substantial reduction in the degree of polymerization of the pulp cellulose. When the reaction was monitored over time, a progressive depolymerization of the cellulose was apparent with the concomitant production of cellobiono-1,5-lactone. Analysis of the reaction filtrates indicated that glucose and arabinose were the only neutral sugars generated. These sugars are derived from the degradation of the cellobiose rather than resulting from modifications of the pulp. These results suggest that the action of cellobiose dehydrogenase results in the generation of hydroxyl radicals via Fenton's chemistry which subsequently results in the depolymerization of cellulose. This appears to be the mechanism whereby a substantial reduction in the degree of polymerization of the cellulose can be achieved without a significant release of sugar.

References

May 23, 1991·European Journal of Biochemistry·G CanevasciniJ L Dreyer
Feb 26, 1991·European Journal of Biochemistry·G HenrikssonE Uzcategui
Sep 13, 1993·Biochimica Et Biophysica Acta·G HenrikssonG Pettersson
Apr 1, 1965·The Biochemical Journal·G HALLIWELL
Dec 1, 1996·Applied and Environmental Microbiology·B P RoyF S Archibald

❮ Previous
Next ❯

Citations

Dec 21, 2002·The Journal of Biological Chemistry·B Martin HallbergChristina Divne
Mar 29, 2008·FEMS Microbiology Reviews·Petr Baldrian, Vendula Valásková
Jul 28, 2010·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Roland LudwigLo Gorton
May 26, 2012·PloS One·Daniel van der LelieSusannah G Tringe
Feb 12, 2013·Applied and Environmental Microbiology·Oleksandr SkybaShawn D Mansfield
May 10, 2011·Applied and Environmental Microbiology·Amber Vanden WymelenbergDan Cullen
Jul 25, 2006·Bioscience, Biotechnology, and Biochemistry·Seiji NakagameJun Sugiura
Jan 11, 2011·Applied and Environmental Microbiology·Wolfgang HarreitherRoland Ludwig
Sep 7, 2000·Microbiology and Molecular Biology Reviews : MMBR·R MaheshwariM K Bhat
Jul 13, 2014·Applied and Environmental Microbiology·Jill GaskellDan Cullen
Nov 28, 2014·Microbiology and Molecular Biology Reviews : MMBR·Fernando SegatoRolf A Prade
Mar 29, 2000·Biochemical and Biophysical Research Communications·B LiV Renganathan
Mar 22, 2000·Journal of Biotechnology·G HenrikssonG Pettersson
Jun 9, 2001·Archives of Biochemistry and Biophysics·F A RotsaertM H Gold
Sep 27, 2000·Biochimica Et Biophysica Acta·G HenrikssonG Johansson
Feb 13, 2001·Enzyme and Microbial Technology·M D. Cameron, S D. Aust
May 20, 1999·Journal of Microbiological Methods·U BamingerD Haltrich
May 29, 2021·Carbohydrate Research·Tamilvendan ManavalanVincent G H Eijsink
Oct 24, 2009·Archives of Biochemistry and Biophysics·Christopher G MowatStephen K Chapman

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.