Cells assemble invadopodia-like structures and invade into matrigel in a matrix metalloprotease dependent manner in the circular invasion assay.

PloS One
Xinzi Yu, Laura Machesky

Abstract

The ability of tumor cells to invade is one of the hallmarks of the metastatic phenotype. To elucidate the mechanisms by which tumor cells acquire an invasive phenotype, in vitro assays have been developed that mimic the process of cancer cell invasion through basement membrane or in the stroma. We have extended the characterization of the circular invasion assay and found that it provides a simple and amenable system to study cell invasion in matrix in an environment that closely mimics 3D invasion. Furthermore, it allows detailed microscopic analysis of both live and fixed cells during the invasion process. We find that cells invade in a protease dependent manner in this assay and that they assemble focal adhesions and invadopodia that resemble structures visualized in 3D embedded cells. We propose that this is a useful assay for routine and medium throughput analysis of invasion of cancer cells in vitro and the study of cells migrating in a 3D environment.

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Citations

Jun 25, 2014·PloS One·Chi-Li ChiuMichelle A Digman
Mar 7, 2014·Proceedings of the National Academy of Sciences of the United States of America·Pei-Hsun WuDenis Wirtz
Oct 24, 2012·The Journal of Cell Biology·Xinzi YuLaura M Machesky
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Datasets Mentioned

BETA
GM6001

Methods Mentioned

BETA
confocal microscopy
Assay

Software Mentioned

Chemotaxis Tool
GeneSnap
ImageJ

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