Cells differentiated from mouse embryonic stem cells via embryoid bodies express renal marker molecules

Differentiation; Research in Biological Diversity
Jan KramerJ Rohwedel

Abstract

Differentiation of mouse embryonic stem (ES) cells via embryoid bodies (EB) is established as a suitable model to study cellular processes of development in vitro. ES cells are known to be pluripotent because of their capability to differentiate into cell types of all three germ layers including germ cells. Here, we show that ES cells differentiate into renal cell types in vitro. We found that genes were expressed during EB cultivation, which have been previously described to be involved in renal development. Marker molecules characteristic for terminally differentiated renal cell types were found to be expressed predominantly during late stages of EB cultivation, while marker molecules involved in the initiation of nephrogenesis were already expressed during early steps of EB development. On the cellular level--using immunostaining--we detected cells expressing podocin, nephrin and wt-1, characteristic for differentiated podocytes and other cells, which expressed Tamm-Horsfall protein, a marker for distal tubule epithelial cells of kidney tissue. Furthermore, the proximal tubule marker molecules renal-specific oxido reductase, kidney androgen-related protein and 25-hydroxyvitamin D3alpha-hydroxylase were found to be expressed ...Continue Reading

References

Mar 1, 1990·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E OosterwijkS O Warnaar
Jan 1, 1989·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·Y NagashimaK Misugi
Aug 1, 1989·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P Ekblom
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A GosslerR Kemler
Jan 1, 1987·Differentiation; Research in Biological Diversity·L Saxén, E Lehtonen
Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·H KawashimaK Kurokawa
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G R Martin
Jul 21, 1995·The Journal of Biological Chemistry·R B ThomsonP S Aronson
Mar 30, 1995·Nature·W Shawlot, R R Behringer
Mar 1, 1995·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K IwataT Setoguchi
Jan 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·T FujiiH Westphal
Jan 1, 1993·Mechanisms of Development·J F ArmstrongJ B Bard
Aug 27, 1993·Cell·J A KreidbergR Jaenisch
Nov 1, 1995·Anatomy and Embryology·P Mundel, W Kriz
Aug 1, 1996·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·L B ZimmerhacklR K Kinne
Mar 1, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·A T Dudley, E J Robertson
Sep 4, 1998·Reproduction, Fertility, and Development·P D RathjenJ Rathjen
Nov 6, 1998·Science·J A ThomsonJ M Jones
Jul 8, 1999·Proceedings of the National Academy of Sciences of the United States of America·V RuotsalainenK Tryggvason
Aug 25, 1999·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·K Pritchard-Jones
Oct 3, 1999·Physiological Reviews·M F HorsterS M Huber
Dec 11, 1999·Cells, Tissues, Organs·J RohwedelA M Wobus
Aug 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·Q YangY S Kanwar
Sep 21, 2000·American Journal of Physiology. Renal Physiology·C LiJ H Miner
May 22, 2001·Stem Cells·J S OdoricoJ A Thomson
Jun 21, 2001·Molecular and Cellular Biology·D B DonovielD R Powell

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Citations

Nov 15, 2007·Stem Cells and Development·Miguel BartheleryKent E Vrana
Apr 23, 2011·Stem Cells and Development·Cristina Fuente MoraPatricia Murray
May 6, 2008·Tissue Engineering. Part C, Methods·Will W MinuthHayo Castrop
Dec 19, 2013·PloS One·Stefano Da SaccoLaura Perin
Aug 29, 2014·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Oliver GrossConstantinos Deltas
Feb 13, 2010·Annual Review of Physiology·Jian-Kan Guo, Lloyd G Cantley
Jul 15, 2015·Expert Opinion on Biological Therapy·Elena LazzeriLaura Lasagni
Feb 8, 2013·Kidney International·Karthikeyan NarayananJackie Y Ying
Jun 9, 2009·Differentiation; Research in Biological Diversity·Cynthia A BatchelderAlice F Tarantal
Oct 11, 2008·Methods : a Companion to Methods in Enzymology·Akira JorakuJames J Yoo
Jul 5, 2007·Journal of Bioscience and Bioengineering·Hiroshi Kurosawa
Nov 5, 2011·Journal of Tissue Engineering and Regenerative Medicine·Shuang Hong LüChang Yong Wang
Feb 9, 2007·Differentiation; Research in Biological Diversity·Stephen J BruceAndrew C Perkins
Apr 23, 2009·Stem Cell Research·Anke M SmitsMarie-José Goumans
Jan 25, 2011·Biochimica Et Biophysica Acta·Heather H WardTamara Roitbak
Apr 27, 2011·Organogenesis·Orit Harari-SteinbergBenjamin Dekel
Oct 6, 2012·Organogenesis·Mara Cananzi, Paolo De Coppi
Jan 3, 2012·Biochemical and Biophysical Research Communications·Masaki NishikawaNorimoto Yanagawa
Mar 11, 2015·BioMed Research International·Marek Karczewski, Tomasz Malkiewicz

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