Cells expressing murine RAD52 splice variants favor sister chromatid repair.

Molecular and Cellular Biology
Peter H ThorpeRodney Rothstein

Abstract

The RAD52 gene is essential for homologous recombination in the yeast Saccharomyces cerevisiae. RAD52 is the archetype in an epistasis group of genes essential for DNA damage repair. By catalyzing the replacement of replication protein A with Rad51 on single-stranded DNA, Rad52 likely promotes strand invasion of a double-stranded DNA molecule by single-stranded DNA. Although the sequence and in vitro functions of mammalian RAD52 are conserved with those of yeast, one difference is the presence of introns and consequent splicing of the mammalian RAD52 pre-mRNA. We identified two novel splice variants from the RAD52 gene that are expressed in adult mouse tissues. Expression of these splice variants in tissue culture cells elevates the frequency of recombination that uses a sister chromatid template. To characterize this dominant phenotype further, the RAD52 gene from the yeast Saccharomyces cerevisiae was truncated to model the mammalian splice variants. The same dominant sister chromatid recombination phenotype seen in mammalian cells was also observed in yeast. Furthermore, repair from a homologous chromatid is reduced in yeast, implying that the choice of alternative repair pathways may be controlled by these variants. In addi...Continue Reading

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Citations

Jul 3, 2008·The Journal of Biological Chemistry·Wataru KagawaShigeyuki Yokoyama
Aug 16, 2008·The Journal of Biological Chemistry·Iben PlateUffe H Mortensen
Dec 30, 2008·The Journal of Biological Chemistry·Karen A UrtishakEric J Brown
Apr 18, 2008·Nucleic Acids Research·Jung-Young ParkYoungho Kim
Mar 31, 2009·BMC Molecular Biology·Aaron M GruverDouglas L Pittman
Sep 21, 2016·Genes·Kritika HanamshetAlexander V Mazin

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