Abstract
Under certain circumstances differentiated cells can be reprogrammed to form stem cells in land plants, but only a portion of the cells reprograms successfully. A long-distance inhibitory signal from reprogrammed cells to surrounding cells has been reported in some ferns. Here we show the existence of anisotropic inhibitory signal to regulate stem cell formation in the moss Physcomitrella patens. When single cells were isolated from a gametophore leaf, over 90% of them were reprogrammed to stem cells with characteristic nuclear expansion. By contrast, when two adjacent cells were isolated, the nuclei of both cells expanded, but successful reprogramming of both cells occurred only in approximately one fifth of the pairs. When three aligned cells were isolated, the reprogramming rate of both edge cells decreased with a living middle cell but did not with a dead middle cell. Furthermore, unequal conversion into stem cells was more prominent in cell pairs aligned parallel to the proximal-distal leaf axis than in those perpendicular to the axis. This study gives an insight into the role of the inhibitory signal in development and evolution as well as the efficient stem cell induction from differentiated cells.
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Citations
Dec 20, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Rumiko KofujiMitsuyasu Hasebe
Mar 6, 2019·ELife·Elena KozgunovaGohta Goshima
Jul 10, 2019·Nature Plants·Masaki IshikawaMitsuyasu Hasebe
Mar 16, 2019·Nucleic Acids Research·Minoru KuboMitsuyasu Hasebe
Oct 28, 2019·Scientific Reports·Elena Kozgunova, Gohta Goshima