Cellular accumulation and activity of quinolones in ciprofloxacin-resistant J774 macrophages.

Antimicrobial Agents and Chemotherapy
Jean-Michel MichotFrançoise Van Bambeke

Abstract

Ciprofloxacin is the substrate for a multidrug resistance-related protein (MRP)-like multidrug transporter in J774 mouse macrophages, which also modestly affects levofloxacin but only marginally affects garenoxacin and moxifloxacin (J.-M. Michot et al., Antimicrob. Agents Chemother. 49:2429-2437, 2005). Two clones of ciprofloxacin-resistant cells were obtained by a stepwise increase in drug concentration (from 34 to 51 to 68 mg/liter) in the culture fluid. Compared to wild-type cells, ciprofloxacin-resistant cells showed (i) a markedly reduced ciprofloxacin accumulation (12% of control) and (ii) a two- to threefold lower sensitivity to the enhancing effect exerted by MRP-inhibitors (probenecid and MK571) on ciprofloxacin accumulation or by ciprofloxacin itself. ATP-depletion brought ciprofloxacin accumulation to similarly high levels in both wild-type and ciprofloxacin-resistant cells. Garenoxacin and moxifloxacin accumulation remained unaffected, and levofloxacin showed an intermediate behavior. DNA and protein synthesis were not impaired in ciprofloxacin-resistant cells for ciprofloxacin concentrations up to 100 mg/liter (approximately 85 and 55% inhibition, respectively, in wild-type cells). In Listeria monocytogenes-infecte...Continue Reading

References

Oct 1, 1990·The Journal of Antimicrobial Chemotherapy·M B CarlierP M Tulkens
Aug 26, 1998·Methods in Enzymology·M M GottesmanI Pastan
Jun 30, 2000·Biochemical Pharmacology·F Van BambekeP M Tulkens
Aug 14, 2001·European Journal of Cell Biology·D TytecaM P Mingeot-Leclercq
Sep 2, 2003·Journal of Leukocyte Biology·Emily ErikssonKristian Riesbeck
Jun 25, 2004·Antimicrobial Agents and Chemotherapy·Jean-Michel MichotPaul M Tulkens

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Citations

Jun 25, 2008·Antimicrobial Agents and Chemotherapy·Solène GrayoAlban Le Monnier
Feb 27, 2008·Antimicrobial Agents and Chemotherapy·S GrayoA Le Monnier
Jan 18, 2008·Journal of Pharmaceutical Sciences·Ana I AlvarezGracia Merino
Jun 13, 2017·International Journal of Molecular Sciences·Mauro CataldiDomenico Capone
Aug 18, 2020·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Ursula TheuretzbacherEmilie Alirol

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