Cellular accumulation and distribution of uranium and lead in osteoblastic cells as a function of their speciation

Toxicology
S MilgramB Gouget

Abstract

Uranium (U) and lead (Pb) are accumulated and fixed for long periods in bone, impairing remodeling processes. Their toxicity to osteoblasts, the cells responsible for bone formation, is poorly documented. It has been previously shown that cytotoxicity and phenotypic effects of both metals on osteoblasts are highly influenced by metal speciation. Differences in sensitivity between cell types have been underlined as well. In this paper, cellular accumulation of U and Pb in cultured and primary osteoblastic cells was assessed by trace element analysis. Distribution of different species at the cell scale was investigated by electron microscopy. Internalization of both metals was shown to be correlated to cytotoxicity and population growth recovery after exposure. For each metal, the amount of metal uptake leading to 50% cell death was shown to be speciation-dependent. Scanning and transmission electron microscopy showed the formation of precipitates with phosphate in lysosomes for both metals, whose role in toxicity or cell defence remains to be clarified. Although a clear link was established between cytotoxicity and accumulation, differences in sensitivity observed in terms of speciation could not be fully explained and other stu...Continue Reading

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Citations

Mar 19, 2013·Metallomics : Integrated Biometal Science·Carole BressonVéronique Malard
Sep 13, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Yann GuéguenChristine Tessier
Mar 17, 2015·Journal of Applied Toxicology : JAT·Shino Homma-TakedaYoshiya Shimada
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Nov 23, 2016·Proceedings of the National Academy of Sciences of the United States of America·Eduardo ParedesCarole Bresson
Oct 13, 2018·Toxicology Letters·Piyawan PaisrisarnSuwimon Boonrungsiman
Nov 12, 2010·Chemical Research in Toxicology·Caroline RouasYann Gueguen

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