Cellular and humoral immune response to recombinant Escherichia coli OmpA in cows

PloS One
Pascal RainardPierre Germon

Abstract

The outer membrane protein (Omp) A is a major constituent of the outer membrane of Escherichia coli. This protein has been used in several vaccine development studies, but seldom with a view to vaccinating against mastitis. The objective of this study was to investigate the immunogenicity of E. coli OmpA and its vaccine potential for cows. Both the humoral and cellular immune responses were investigated. The gene for OmpA of the mastitis-causing strain P4 was cloned and expressed, and the recombinant protein (rEcOmpA) purified. Cows were immunized twice with rEcOmpA with adjuvant one month apart by the systemic route. Before immunization, few antibodies to rEcOmpA were detected, and there was little production of IL-17A in a whole blood stimulation assay (WBA) with rEcOmpA. Antibodies to rEcOmpA were induced by immunization. These antibodies were not able to react with E. coli P4, but reacted with a rough P4 mutant prepared by inactivating the rfb locus. This suggests that the complete LPS O-chain precluded the accessibility of antibodies to their target at the outer membrane. The cellular immune response appeared to be biased towards a Th17-type, as more IL-17A than IFN-γ was produced in the OmpA-specific WBA. There was a good...Continue Reading

References

Sep 1, 1987·Journal of Bacteriology·W J RocqueE J McGroarty
Apr 5, 1986·Journal of Molecular Biology·R FreudlU Henning
Oct 1, 1985·Veterinary Immunology and Immunopathology·N Craven, M R Williams
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Aug 10, 2000·Molecular Microbiology·R KoebnikP Van Gelder
Mar 22, 2002·Biochemical and Biophysical Research Communications·Ying Wang
Jul 3, 2002·Nature Reviews. Immunology·Raymond M Welsh, Liisa K Selin
Jun 15, 2007·FEMS Microbiology Letters·Stephen G J SmithRobert P Fagan
Dec 7, 2010·Protein Expression and Purification·Michael J McConnell, Jerónimo Pachón
Jan 14, 2012·The FEBS Journal·Subramanian Krishnan, Nemani V Prasadarao
Sep 19, 2012·Veterinary Microbiology·Anthony W Confer, Sahlu Ayalew
May 7, 2013·Trends in Immunology·Yasmine BelkaidTimothy W Hand
Jan 9, 2015·Applied Microbiology and Biotechnology·Qingfeng GuanJianhua Wang
Dec 20, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Adeline PorcheriePascal Rainard
Apr 23, 2016·PloS One·Pascal RainardFlorence B Gilbert
Mar 25, 2017·Frontiers in Immunology·Marisol Pérez-ToledoConstantino López-Macías
Jun 15, 2017·Scientific Reports·Vincent HerryPascal Rainard

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Methods Mentioned

BETA
PCR
ELISA
ELISAs
flow cytometry

Software Mentioned

GraphPad Prism
XLSTAT

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