PMID: 7514153May 15, 1994Paper

Cellular and karyotypic characterization of two doxorubicin resistant cell lines isolated from the same parental human leukemia cell line

International Journal of Cancer. Journal International Du Cancer
J R ZalcbergL Campbell

Abstract

Separate mechanisms underlying the multidrug resistant (MDR) phenotype were identified in 2 independent approaches to select tumour cells resistant to low concentrations of doxorubicin (Dox) from the sensitive T cell leukemia cell line CCRF-CEM. The CEM/A7 cell line was selected at an initial concentration of 0.005 microgram/ml of Dox and maintained at 0.07 microgram/ml. In contrast, the CEM/A5 line was selected using an initial concentration of 0.01 microgram/ml and maintained in Dox at a concentration of 0.05 microgram/ml. P-glycoprotein expression was demonstrated in the CEM/A7 line but not the CEM/A5 line. Amplification of the mdrI gene was not observed in the CEM/A7 cell line. Both cell lines showed cross-resistance to a number of structurally unrelated cytotoxic drugs including anthracyclines and etoposide (VP-16), although only the CEM/A7 line was cross resistant to Vinca alkaloids. Immunoblots of total cell lysates of the CEM/A5 line have revealed almost undetectable levels of topoisomerase II alpha and beta in this line. Cytogenetic analyses of both lines revealed numerous karyotypic abnormalities which were present in the parental cell line as well as both resistant cell lines. The CEM/A7 line also demonstrated a dupl...Continue Reading

References

Jul 7, 1992·Biochimica Et Biophysica Acta·D Nielsen, T Skovsgaard
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M Tsai-PflugfelderJ C Wang
Feb 1, 1983·Cancer Genetics and Cytogenetics·L M Webber, O M Garson

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Citations

Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·M J SmythR W Johnstone
Sep 28, 2000·Biochemical and Biophysical Research Communications·K M TaintonR W Johnstone
Apr 17, 2001·Biochemical and Biophysical Research Communications·L Shearwin-WhyattS Kumar
Mar 29, 2000·Biochemical Pharmacology·M Vezmar, E Georges
Dec 31, 2008·Journal of Experimental & Clinical Cancer Research : CR·Leticia Oliveira-FerrerGunter Schuch
Jul 9, 2010·PloS One·Sven R KantelhardtDonna J Arndt-Jovin
Mar 30, 2001·The Journal of Biological Chemistry·R W JohnstoneM J Smyth

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