Cellular and molecular immune responses of the sea bass (Dicentrarchus labrax) experimentally infected with betanodavirus

Fish & Shellfish Immunology
G ScapigliatiM C Alvarez

Abstract

Naïve sea bass juveniles (38.4 + or - 4.5 g) were intramuscularly infected with a sublethal dose of betanodavirus isolate 378/I03, followed after 43 days by a similar boosting. This infection resulted in an overall mortality of 7.6%. At various intervals, sampling of fish tissues was performed to investigate: i) B and T lymphocyte content in organs and tissues; ii), proliferation of leucocytes re-stimulated in vitro with inactivated virus; iii) presence of serum antibody specific for betanodavirus; iv) expression of genes coding for the following immunoregulatory molecules involved in innate and acquired responses: type I IFN, Mx, IL-1, Cox-2; IL-10, TGF-beta, TCRbeta, CD4, CD8alpha, IgM, by using a quantitative PCR array system developed for sea bass. The obtained results showed a detectable increase of T cells and B cells in PBL during betanodavirus infection. Furthermore, leucocytes obtained from blood, head kidney, and gills showed a detectable "in vitro" increase in viability upon addition of inactivated viral particles, as determined by measuring intracellular ATP concentration. ELISA analysis of sera showed that exposure to nodavirus induced a low, but specific antibody titer measured 43 days after infection, despite the...Continue Reading

Citations

Apr 4, 2013·Veterinary Immunology and Immunopathology·P NovelJ Béjar
Aug 7, 2013·Developmental and Comparative Immunology·Young-Mao ChenTzong-Yueh Chen
Sep 20, 2015·Fish & Shellfish Immunology·Suhee HongHyun Do Jeong
Dec 26, 2015·Preventive Veterinary Medicine·Diana JaramilloRichard Whittington
Apr 14, 2012·Analytical Biochemistry·Marie-Aline PierrardFrédéric Silvestre
Apr 17, 2012·Developmental and Comparative Immunology·Ming-Shan WuChi-Yao Chang
Mar 1, 2011·Developmental and Comparative Immunology·Fumio TakizawaUwe Fischer
Sep 17, 2016·Journal of Fish Diseases·Q K DoanF Allal
Feb 14, 2020·Pathogens·Isabel Bandín, Sandra Souto
Oct 4, 2017·PeerJ·Chean Yeah YongWen Siang Tan
Apr 17, 2021·Frontiers in Veterinary Science·Henrike SeibelAlexander Rebl
Oct 25, 2011·Developmental and Comparative Immunology·Aina-Cathrine OvergårdSonal Patel

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