Feb 1, 1991

Cellular and regional distribution of reduced glutathione in the nervous system of the rat: histochemical localization by mercury orange and o-phthaldialdehyde-induced histofluorescence

Toxicology and Applied Pharmacology
Martin A PhilbertHerbert Lowndes

Abstract

Differences in the cellular distribution of antioxidant defense mechanisms in heterogeneous tissue such as the nervous system are likely critical determinants of differential sensitivity to toxicants. Regional and cellular localization of reduced glutathione (GSH) in central and peripheral nervous tissue was determined from the pattern of fluorescence observed in tissue sections stained with mercury orange; localization was confirmed using a novel histofluorochromatic staining method, o-phthaldialdehyde (OPT). Excellent concordance between the distribution of fluorescence obtained with mercury orange and OPT staining was observed. Depletion of GSH by treatment with diethyl maleate resulted in a diminution in both mercury orange and OPT histofluorescence. Generally, strong staining of the CNS neuropil was seen with little or no observable fluorescence in neuronal somata. The cerebellar granular cells were an exception, exhibiting fluorescence with both mercury orange and OPT. Cerebellar Purkinje cells exhibited nonuniform fluorescence with mercury orange but generally uniform staining with OPT. In contrast to the patterns observed in the CNS, the sciatic nerve and the sensory cell bodies of the lumbar dorsal root ganglia exhibit...Continue Reading

  • References18
  • Citations59

Mentioned in this Paper

Establishment and Maintenance of Localization
Portion of Heterogeneous Tissue
Structure of Sciatic Nerve
Neuropil
Phenylmercury Compounds
Tract
Enzymes, antithrombotic
August Rats
Entire Nervous System
Brain

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