Cellular and subcellular localization of delta opioid receptor immunoreactivity in the rat dentate gyrus

Brain Research
K Commons, Teresa A Milner

Abstract

To study a potential locus of action of opioids in the rat dentate gyrus, we examined the localization of the delta opioid receptor (DOR) by immunocytochemistry. Two antisera raised to unique, non-overlapping peptide sequences located within the extracellular N-terminal sequence of DOR were tested. By light microscopy, numerous neurons in the central hilar region were intensely labeled for DOR, while the granule cell layer contained light DOR immunoreactivity. To further characterize hilar neuron cell types which contained DOR, sections through the dentate gyrus were double labeled using immunofluorescence with antisera to DOR and either gamma-aminobutyric acid (GABA), neuropeptide Y (NPY), or somatostatin-28 antisera. Most DOR-labeled perikarya also contained GABA and NPY, while a subpopulation contained somatostatin. Electron microscopic examination of sections labeled for DOR revealed that the immunoreactivity was common in profiles which exhibited the morphological characteristics of granule cells, as well as those of non-granule cells. DOR immunoreactivity was located at postsynaptic sites within neuronal perikarya (2%), dendrites (27%), and dendritic spines (22%); as well as in presynaptic axon terminals (25%) and glia (2...Continue Reading

Citations

Jan 19, 2012·Cellular and Molecular Neurobiology·Xavier RezaïDominique Massotte
May 9, 2002·American Journal of Physiology. Cell Physiology·Junhui ZhangYing Xia
Aug 22, 2017·Frontiers in Molecular Neuroscience·Johannes Burtscher, Christoph Schwarzer
Mar 27, 1999·Molecular Microbiology·B P GuoM Höök
Aug 25, 2007·Behavioral and Cognitive Neuroscience Reviews·Mikhail A Lebedev, Steven P Wise
Jun 19, 2004·The International Journal of Neuroscience·Joseph E Bogen
Jan 3, 2001·The Journal of Comparative Neurology·A L Barker-GibbT A Milner
May 3, 2006·Cells, Tissues, Organs·Pansiri Phansuwan-PujitoPiyarat Govitrapong
Sep 29, 2017·Physiological Reviews·Kenneth A PelkeyChris J McBain
Dec 11, 2019·Neuroscience and Biobehavioral Reviews·Corey B PuryearConnie Sanchez
Mar 21, 2006·Life Sciences·Andrew C GrayPaul J White

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.