PMID: 9161849Mar 1, 1997Paper

Cellular antioxidant properties of human natural killer enhancing factor B

Free Radical Research
T A SarafianHungyi Shau

Abstract

The protein, NKEF (natural killer enhancing factor), has been identified as a member of an antioxidant family of proteins capable of protecting against protein oxidation in cell-free assay systems. The mechanism of action for this family of proteins appears to involve scavenging or suppressing formation of protein thiyl radicals. In the present study we investigated the antioxidant protective properties of the NKEF-B protein overexpressed in an endothelial cell line (ECV304). Nkef-B-transfected cells displayed significantly lower levels of reactive oxygen species (ROS) compared with control or vector-transfected cells. Tert-Butylhydroperoxide-induced ROS was 15% lower in nkef-B-transfected cells and cytotoxicity was slightly, though not significantly, lower. NKEF-B had no effect on ROS induced by menadione or xanthine plus xanthine oxidase. NKEF-B overexpression resulted in slightly (approximately 10%) lower levels of cellular glutathione (GSH) and had no effect on rate or extent of GSH depletion following either diethylmaleate (DEM) or buthionine sulfoximine (BSO) treatment. Lipid peroxidation, assessed as thiobarbituric acid-reactive substances, was 40% lower in nkef-B-transfected cells compared with vector-only-transfected c...Continue Reading

References

Nov 1, 1992·Journal of Neurochemistry·B Halliwell
Mar 1, 1991·The International Journal of Neuroscience·J A Jesberger, J S Richardson
Feb 23, 1989·Nature·J M Gallo, B H Anderton
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·I H KimS G Rhee
Mar 28, 1995·Biochemical and Biophysical Research Communications·H SauriH Shau
May 30, 1994·Biochemical and Biophysical Research Communications·S J KwonK Kim
Feb 28, 1994·Biochemical and Biophysical Research Communications·H Shau, A Kim
Sep 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·B N AmesT M Hagen
Nov 1, 1995·Free Radical Biology & Medicine·J T NaaralaK M Savolainen
May 1, 1996·Journal of Neuropathology and Experimental Neurology·S S Chin, J E Goldman
Jan 1, 1996·Free Radical Biology & Medicine·H F GalleyN R Webster

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Citations

Oct 21, 1998·Molecular and Chemical Neuropathology·T A SarafianH Shau
Mar 10, 2001·Antioxidants & Redox Signaling·L H ButterfieldH Shau
May 26, 1999·American Journal of Respiratory Cell and Molecular Biology·T A SarafianM D Roth
Dec 2, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jenn-Han ChenYu-Ju Chen
Sep 24, 1999·Journal of Neuroscience Research·T A SarafianH Shau
Nov 17, 2005·Cancer Research·Tieli WangJian Jian Li

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