PMID: 9488223Mar 6, 1998

Cellular changes induced by chronic nitric oxide inhibition in intact rat basilar arteries revealed by confocal microscopy

Journal of Hypertension
Silvia M ArribasJohn C McGrath


Cellular aspects of remodelling have not been investigated fully in intact vessels due to lack of appropriate methodology. To determine the cellular alterations induced by chronic inhibition of nitric oxide (NO) production in intact rat basilar arteries by combined use of perfusion myography and a laser scanning confocal microscope. Wistar-Kyoto rats were treated with 10 mg/kg per day NG-nitro-L-arginine methyl ester (L-NAME) for 3 weeks. Basilar arteries from treated and age-matched Wistar-Kyoto rat controls were mounted on a perfusion myograph, stained with the nuclear dye Hoechst 33342 and fixed under pressure. The segments were mounted on a slide and visualized using the 364 nm line of a laser scanning confocal microscope. MetaMorph software was used to obtain optical sections from the vessel and for morphology determinations. L-NAME treatment induced hypertension (systolic blood pressure control 129.2+/-2.7 mmHg and SBP L-NAME treatment 176.3+/-5.2 mmHg, P< 0.001). Compared with control rat arteries, arteries from treated rats had a reduced lumen diameter, similar wall thickness and an increased wall: lumen ratio. L-NAME treatment induced specific changes in adventitia, media and intima, namely an increase in number of adv...Continue Reading


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Related Concepts

Muscle, Smooth, Vascular
Tunica Intima
Arterial System
Entire Lumen of Body System
Systolic Blood Pressure Measurement
Science of Morphology
Myocytes, Smooth Muscle
Rats, Inbred WKY
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