Abstract
Immunotherapy of gynaecological cancer with tumour-infiltrating lymphocytes (TIL) or peripheral blood lymphocytes (PBL) has become a valid treatment modality with varying degrees of success in obtaining an antitumour response. TIL consist of lymphocytes, mainly T cells and minor populations of natural killer cells or B cells. Conventional cytogenetic studies of tumour cells from patients with breast and ovarian cancer have shown multiple chromosomal abnormalities including chromosomes 7 and 12. This study was designed to analyse the surface further, as well as investigate the intracellular, characteristics of TIL by multicolour flow cytometry and the cytogenetic features by fluorescence in situ hybridization. Tumour cell, peripheral blood and TIL samples from 25 patients (15 ovarian tumours, 8 breast cancers, 1 uterine sarcoma, 1 cervical carcinoma) were analysed for their phenotype, the expression of major cytokines [interleukin-2 (IL-2), IL-4 and interferon gamma (IFN gamma)], their proliferation rate, their cytotoxic ability and for the presence of numerical aberrations of chromosomes 7 and 12. All the tumour cells showed a high frequency of numerical aberration in chromosomes 7 and 12, especially trisomies or tetrasomies an...Continue Reading
Citations
Mar 16, 2000·Cancer·B AbendsteinA G Zeimet
May 13, 2005·Cancer Immunology, Immunotherapy : CII·Koichi SakakuraNobuhiko Furuya
Feb 19, 2004·Human Immunology·Katarína PolákováGustáv Russ
May 26, 1999·Pharmacology & Therapeutics·D W Miles, J Taylor-Papadimitriou
Feb 13, 2002·Journal of Reproductive Immunology·Daniel T ReinHeike Engel
Mar 19, 1999·Immunology Today·J O'ConnellF Shanahan
May 7, 1999·International Journal of Immunopharmacology·J W Hadden
Dec 9, 2003·The Breast : Official Journal of the European Society of Mastology·Stephanie VgenopoulouPanayiotis S Davaris
Jul 20, 2001·Thorax·K TakahashiY Monden
Mar 17, 2012·Breast Cancer Research : BCR·Shuzhen LiuTorsten O Nielsen
Jul 5, 2005·Expert Opinion on Investigational Drugs·D W Miles, J Taylor-Papadimitriou
Jul 29, 2008·The Breast : Official Journal of the European Society of Mastology·Bor-Ching SheuSong-Nan Chow
Jun 2, 1998·Cancer Treatment Reviews·T A Plunkett, D W Miles
Sep 30, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Susan K LutgendorfDavid M Lubaroff
Sep 17, 1999·São Paulo Medical Journal = Revista Paulista De Medicina·B M Tavares MurtaE F Murta
Nov 15, 2006·Head & Neck·Kazuaki ChikamatsuNobuhiko Furuya
Apr 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Concetta ConticelloRuggero De Maria
Jul 3, 1999·Clinical and Diagnostic Laboratory Immunology·J O'ConnellF Shanahan