Cellular characterization of human epicardial adipose tissue: highly expressed PAPP-A regulates insulin-like growth factor I signaling in human cardiomyocytes.

Physiological Reports
Cheryl A ConoverHartzell V Schaff

Abstract

Little is known about the cellular biology of fat surrounding the human heart. In this study, we obtained paired samples of epicardial fat, the visceral fat depot attached to the heart, and subcutaneous skin fat from patients undergoing open heart surgery to test the hypothesis that human epicardial fat cells differentially express bioactive molecules that have the potential to affect cardiac function. First, we characterized the free fatty acids (FFAs), adipocytokines, and growth factors secreted by isolated adipocytes and preadipocytes in cell culture. There was little to distinguish the fat cell secretory products in terms of FFAs and adipocytokines. The most striking finding was that preadipocytes from epicardial adipose tissue expressed high levels of pregnancy-associated plasma protein-A (PAPP-A), a novel metalloproteinase that enhances local insulin-like growth factor (IGF) action through cleavage of inhibitory IGF binding protein-4 (IGFBP-4). PAPP-A levels were 15-fold higher in conditioned medium from epicardial preadipocytes than from subcutaneous preadipocytes (P < 0.0001). PAPP-A was not expressed in mature adipocytes. Next we determined whether PAPP-A could affect IGF-I signaling in a human cardiomyocyte cell line....Continue Reading

References

Feb 13, 2003·Obesity Research·Gianluca IacobellisFrida Leonetti
Jun 5, 2003·Journal of Lipid Research·Yourka D TchoukalovaMichael D Jensen
Oct 29, 2003·Circulation·Tomasz MazurekYi Shi
Apr 6, 2005·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Ole Saetrum Opgaard, Ping H Wang
Sep 21, 2006·American Journal of Physiology. Endocrinology and Metabolism·Tamara TchkoniaJames L Kirkland
Jun 2, 2007·American Heart Journal·Harold S Sacks, John N Fain
Jan 2, 2008·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Anilkumar K ReddyGeorge E Taffet
Apr 25, 2008·The Journal of Biological Chemistry·Jakob H MikkelsenClaus Oxvig
Mar 17, 2009·Biochemical and Biophysical Research Communications·Yourka D TchoukalovaEric Ravussin
Mar 31, 2010·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Cheryl A ConoverRonald J Marler
Apr 17, 2010·Arteriosclerosis, Thrombosis, and Vascular Biology·Kalypso KarastergiouVidya Mohamed-Ali
Jun 19, 2010·Circulation. Arrhythmia and Electrophysiology·George ThanassoulisEmelia J Benjamin
Aug 19, 2010·Journal of Cellular and Molecular Medicine·D Margriet OuwensJuergen Eckel
Nov 26, 2010·International Journal of Cardiology·Gianluca IacobellisArya M Sharma
Aug 20, 2011·Trends in Endocrinology and Metabolism : TEM·Gianluca Iacobellis, Antonio C Bianco
Oct 1, 2011·Journal of the American College of Cardiology·Manu Kaushik, Yeruva M Reddy
Apr 3, 2012·Trends in Endocrinology and Metabolism : TEM·Cheryl A Conover
Apr 16, 2013·Cell Metabolism·Tamara TchkoniaJames L Kirkland
Sep 21, 2013·American Journal of Physiology. Endocrinology and Metabolism·Cheryl A ConoverJames L Kirkland
Oct 12, 2013·Obesity·Gianluca IacobellisGiuseppe Barbaro
Nov 16, 2013·Journal of cardiology·Tadateru IwayamaIsao Kubota
Jan 2, 2014·Trends in Endocrinology and Metabolism : TEM·Rodrigo TroncosoSergio Lavandero
Mar 7, 2014·Journal of the American Heart Association·Timothy P Fitzgibbons, Michael P Czech
May 2, 2014·The Journal of Endocrinology·Caroline Davidge-PittsCheryl A Conover
Apr 9, 2015·Nature Reviews. Endocrinology·Gianluca Iacobellis
Jan 7, 2016·Journal of Cardiovascular Translational Research·Cheryl A ConoverClaus Oxvig
Aug 16, 2016·The Journal of Endocrinology·Diane DoneganCheryl A Conover
Sep 3, 2016·European Journal of Endocrinology·Mette Faurholdt GudeJan Frystyk
Mar 15, 2018·Journal of Molecular Endocrinology·Fumihiko Hakuno, Shin-Ichiro Takahashi
Jul 20, 2018·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Laurie K BaleCheryl A Conover

❮ Previous
Next ❯

Citations

Sep 3, 2020·The Journal of Clinical Endocrinology and Metabolism·Rikke HjortebjergJo G R De Mey
Sep 6, 2020·Endocrinology·Akhila RamakrishnaCheryl A Conover

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
Assay
ELISAs

Software Mentioned

/ Counting
Analyze
COR
LI

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Related Papers

Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society
Laurie K BaleCheryl A Conover
Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
Sharon J TannerCheryl A Conover
Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society
H B Boldt, Cheryl A Conover
Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
Christopher O OrtizCheryl A Conover
Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society
Cheryl A ConoverLaurie K Bale
© 2022 Meta ULC. All rights reserved