PMID: 9658073Jul 11, 1998Paper

Cellular components interact with adenovirus type 5 minimal DNA packaging domains

Journal of Virology
S I Schmid, Patrick Hearing

Abstract

Adenovirus type 5 DNA packaging is initiated from the left end of the viral genome and depends on the presence of a cis-acting packaging domain located between nucleotides 194 and 380. Multiple redundant packaging elements (termed A repeats I through VII [AI through AVII]) are contained within this domain and display differential abilities to support DNA packaging in vivo. The functionally most important repeats, AI, AII, AV, and AVI, follow a bipartite consensus motif exhibiting AT-rich and CG-rich core sequences. Results from previous mutational analyses defined a fragment containing AV, AVI, and AVII as a minimal packaging domain in vivo, which supports a functional independence of the respective cis-acting sequences. Here we describe multimeric versions of individual packaging elements as minimal packaging domains that can confer viability and packaging activity to viruses carrying gross truncations within their left end. These mutant viruses directly rate the functional role that different packaging elements play relative to each other. The A repeats are likely to be binding sites for limiting, trans-acting packaging factors of cellular and/or viral origin. We report here the characterization of two cellular binding activi...Continue Reading

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Citations

Oct 24, 2003·The Journal of General Virology·Li XingSuresh Kumar Tikoo
Feb 15, 2005·Journal of Virology·Philomena OstapchukPatrick Hearing
Jul 1, 2006·Journal of Virology·Philomena OstapchukPatrick Hearing
Mar 1, 2003·Journal of Virology·Wei Zhang, Michael J Imperiale
Apr 15, 2003·Journal of Virology·Philomena Ostapchuk, Patrick Hearing
Jan 19, 2007·Journal of Virology·Ryan E TylerMichael J Imperiale
Oct 11, 2016·Frontiers in Microbiology·Yadvinder S Ahi, Suresh K Mittal
Oct 3, 2001·Journal of Virology·W ZhangM J Imperiale
Oct 9, 2002·Journal of Virology·Manuel A F V GonçalvesAntoine A F de Vries
May 20, 2006·Reviews in Medical Virology·Manuel A F V Gonçalves, Antoine A F de Vries
Nov 2, 2001·Biological Chemistry·T DobnerH H Niller
May 14, 2003·Journal of Virology·Ece ErturkPatrick Hearing
Apr 29, 2011·Gene Therapy·M Gonzalez-AparicioR Hernandez-Alcoceba
Feb 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·V SandigC T Caskey
Jul 1, 2005·Journal of Cellular Biochemistry·Philomena Ostapchuk, Patrick Hearing
Feb 23, 2000·Journal of Virology·W Zhang, M J Imperiale
Mar 22, 2002·Journal of Virology·Thomas PietschmannRalf Bartenschlager
Apr 26, 2001·Molecular Therapy : the Journal of the American Society of Gene Therapy·C SoudaisE J Kremer

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