Cellular considerations for optimizing bone cell culture and remodeling in a lab-on-a-chip platform.

BioTechniques
Sharon L TruesdellMarnie M Saunders

Abstract

Our lab has developed a lab-on-a-chip platform for bone remodeling that enables long-term culturing of bone cells out to 7 weeks and serves as a foundation toward a multicellular organ-on-a-chip system. Here, we optimized culturing protocols for osteoblasts, osteoclasts and osteocytes within the lab-on-a-chip and performed functional activity assays for quantifying bone formation and resorption. We analyzed cell seeding densities, feeding schedules and time in culture as a basis for optimizing culturing protocols. Further, we addressed concerns of sterility, cytotoxicity and leakage during the extended culture period within the polydimethylsiloxane chip. This system provides a method for quantifying the soluble effects of mechanically stimulated osteocytes on bone remodeling (formation/resorption).

References

Mar 29, 2002·Biotechnology and Bioengineering·Mark J PowersLinda G Griffith
Apr 19, 2003·Journal of Colloid and Interface Science·Kirill EfimenkoJan Genzer
Dec 31, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·James G McGarryPatrick J Prendergast
Feb 8, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Hongke YeDavid H Gracias
Jun 26, 2010·Science·Dongeun HuhDonald E Ingber
Aug 18, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Christopher PriceLiyun Wang
Jan 22, 2011·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Lynda F Bonewald
Apr 26, 2011·Biochemical and Biophysical Research Communications·Songzi KouImshik Lee
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Oct 23, 2012·Bone·Jenneke Klein-NulendSheldon Weinbaum
Feb 26, 2015·Biomicrofluidics·Chao WeiJian Chen
Sep 30, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yexin GuWoo Y Lee
Sep 21, 2016·BoneKEy Reports·Karan M ShahAstrid D Bakker
Nov 28, 2017·Drug Metabolism and Pharmacokinetics·Hiroshi KimuraTeruo Fujii
Mar 3, 2018·Experimental Cell Research·Estee L GeorgeMarnie M Saunders
Jan 1, 2012·Journal of Biomimetics, Biomaterials, and Tissue Engineering·Spencer L YorkMarnie M Saunders

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Citations

Jul 23, 2020·Frontiers in Physiology·Lauren RichardsonArum Han

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Methods Mentioned

BETA
chip
chips
biopsy
scanning electron microscopy

Software Mentioned

ImageJ
NIH ImageJ

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