Cellular dielectrophoresis coupled with single-cell analysis

Analytical and Bioanalytical Chemistry
Min Li, Robbyn K Anand

Abstract

In this review, recent advances that leverage dielectrophoretic approaches to accomplish single-cell analysis (both "on-chip" and "off-chip") are discussed with special emphasis on eukaryotic cells. Dielectrophoresis as an electric-field-induced force utilized for cell manipulation can confer selectivity without labeling. Recent technical improvements have increased the volumetric throughput of the separation of cells from complex mixtures, introduced new strategies for massively parallel single-cell confinement for subsequent on-chip analysis, made possible selective transport of individual cells off-chip, and integrated preconcentration and prefocusing steps to enhance dielectrophoretic performance. Collectively, these studies potentiate all-in-one platforms capable of taking as their input complex mixtures of cells and accomplishing single-cell analysis. Assays requiring small reaction volumes (e.g., enzymatic assays, fluorescent in situ hybridization, and immunostaining) have been demonstrated. Still greater opportunities to unravel cell-to-cell variations and for point-of-care applications can be realized by making possible on-chip gene amplification, live-cell assays, and either dielectrophoretic manipulation in native me...Continue Reading

References

Sep 2, 1999·Journal of Colloid and Interface Science·A RamosA Castellanos
Sep 5, 2002·Electrophoresis·Peter R C Gascoyne, Jody Vykoukal
Sep 21, 2002·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·N G GreenA Castellanos
Oct 23, 2003·Analytical Chemistry·Aaron R WheelerAntoine Daridon
Oct 27, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·W Jeffrey AllardLeon W M M Terstappen
May 11, 2005·Nature Reviews. Genetics·Mads KaernJames J Collins
Sep 1, 2005·Analytical Chemistry·Jacqueline R Rettig, Albert Folch
Jul 28, 2006·Nature·Jamil El-AliKlavs F Jensen
Aug 31, 2006·Biotechnology Journal·Ruei-Zeng LinHwan-You Chang
Oct 27, 2006·Lab on a Chip·Dino Di CarloLuke P Lee
Jun 1, 2007·Lab on a Chip·Dirk R AlbrechtSangeeta N Bhatia
Aug 24, 2007·Lab on a Chip·Nikhil MittalJoel Voldman
Oct 5, 2007·Biomedical Microdevices·Hong Miao JiLevent Yobas
Apr 12, 2008·Nature Reviews. Cancer·Klaus PantelBurkhard Brandt
Mar 24, 2009·Electrophoresis·Peter R C GascoyneFrederick F Becker
May 6, 2009·Biomedical Microdevices·Hadi ShafieeRafael V Davalos
Jul 7, 2009·Analytical and Bioanalytical Chemistry·C ZhangK Kalantar-Zadeh
Nov 19, 2009·Molecular Systems Biology·Theodore J Perkins, Peter S Swain
Feb 4, 2010·Lab on a Chip·Hadi ShafieeRafael V Davalos
Apr 27, 2010·Analytical and Bioanalytical Chemistry·Daniel R GossettDino Di Carlo
May 4, 2010·Annual Review of Biomedical Engineering·Richard N Zare, Samuel Kim
Aug 4, 2010·Proceedings of the National Academy of Sciences of the United States of America·Antoine-Emmanuel SalibaJean-Louis Viovy
Oct 12, 2010·Biosensors & Bioelectronics·Khashayar KhoshmaneshKourosh Kalantar-zadeh
Oct 23, 2010·Analytical and Bioanalytical Chemistry·Soumya K SrivastavaAdrienne R Minerick
Apr 14, 2011·Chemical Society Reviews·Maureen A Walling, Jason R E Shepard
Jun 30, 2011·Lab on a Chip·Thomas GerhardtHongshen Ma
Sep 17, 2011·Electrophoresis·Barbaros Cetin, Dongqing Li
Sep 17, 2011·Electrophoresis·Erin A HensleeRafael V Davalos
Oct 1, 2011·Lab on a Chip·Andrew C HatchAbraham P Lee
Dec 3, 2011·Current Opinion in Biotechnology·Huabing Yin, Damian Marshall
Dec 19, 2012·Journal of the American Chemical Society·Joshua P GuerretteBo Zhang
Dec 26, 2012·The Journal of Molecular Diagnostics : JMD·Yi DongDenis A Smirnov
Jan 26, 2013·Electrophoresis·Rodrigo Martinez-DuarteAlexandra Ros
Mar 19, 2013·Analytical Chemistry·Thomas SchneiderDaniel T Chiu

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Citations

Apr 2, 2020·International Journal of Molecular Sciences·Denis V VoroninDaniil N Bratashov
Dec 7, 2019·Electrophoresis·Junyan ZhangYongxin Song
Oct 4, 2020·Sensors·Thomas ChalklenSohini Kar-Narayan
Nov 25, 2020·Analytical Chemistry·Kira L Rahn, Robbyn K Anand
Nov 30, 2018·Analytical Chemistry·Daihyun KimAlexandra Ros

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