Cellular differentiation and I-FABP protein expression modulate fatty acid uptake and diffusion

The American Journal of Physiology
Barbara P AtshavesF Schroeder

Abstract

The effect of cellular differentiation on fatty acid uptake and intracellular diffusion was examined in transfected pluripotent mouse embryonic stem (ES) cells stably expressing intestinal fatty acid binding protein (I-FABP). Control ES cells, whether differentiated or undifferentiated, did not express I-FABP. The initial rate and maximal uptake of the fluorescent fatty acid, 12-(N-methyl)-N-[(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-octadec anoic acid (NBD-stearic acid), was measured in single cells by kinetic digital fluorescence imaging. I-FABP expression in undifferentiated ES cells increased the initial rate and maximal uptake of NBD-stearic acid 1.7- and 1.6-fold, respectively, as well as increased its effective intracellular diffusion constant (Deff) 1.8-fold as measured by the fluorescence recovery after photobleaching technique. In contrast, ES cell differentiation decreased I-FABP expression up to 3-fold and decreased the NBD-stearic acid initial rate of uptake, maximal uptake, and Deff by 10-, 4.7-, and 2-fold, respectively. There were no significant differences in these parameters between the differentiated control and differentiated I-FABP-expressing ES cell lines. In summary, differentiation and expression of I-FA...Continue Reading

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Citations

Aug 24, 2001·The Journal of Clinical Investigation·M SjöblomG Flemström
Aug 15, 2002·American Journal of Physiology. Cell Physiology·Barbara P AtshavesFriedhelm Schroeder
Jun 3, 2005·Zoological Science·Mina Nakauchi, Norio Suzuki
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Mar 10, 1999·The American Journal of Physiology·C DarimontA de Pover
Aug 11, 2000·Clinical and Experimental Hypertension : CHE·V A Lucas-TeixeiraP Soares-da-Silva
May 3, 2001·The Journal of Biological Chemistry·B P AtshavesF Schroeder
Dec 5, 2009·Molecular and Cellular Biochemistry·Nirmalya BasuSandhya S Visweswariah
May 25, 2004·The Journal of Biological Chemistry·Barbara P AtshavesFriedhelm Schroeder
Dec 24, 2008·Molecular and Cellular Biochemistry·Gregory G MartinFriedhelm Schroeder
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Jul 26, 2012·Clinical Pharmacology and Therapeutics·D A KatzkaM Camilleri
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Aug 24, 2000·The Journal of Biological Chemistry·B P AtshavesF Schroeder
Sep 26, 2000·American Journal of Physiology. Cell Physiology·O StarodubF Schroeder
Feb 9, 2012·Histochemistry and Cell Biology·Karen Schwabe, Yalcin Cetin
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Mar 13, 2009·Progress in Lipid Research·Isabelle NiotPhilippe Besnard

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