Cellular distribution patterns of lanthanum and morphometry of rat hearts exposed to different degrees of ischemic stress

The Anatomical Record
A SchmiedlJ Richter

Abstract

The element lanthanum (La) can be used as a tracer for verification of membrane permeability. The aim of this study was to establish whether 1) distribution of La in the myocardium of rat hearts depends on the degree of ischemic stress and 2) morphometrically determined cell and mitochondrial swelling correlates with the La distribution. Isolated beating rat hearts were arrested by coronary perfusion with the cardioplegic solution Custodiol (controls) or by aortic cross clamping followed by exposure to different degrees of ischemic stress. The solutions for perfusion-and postfixation as well as for rinsing contained 1.1% La(NO3)3. Cellular and mitochondrial swelling were determined morphometrically and myocytes exhibiting intracellular La were quantified and stated as percentage of test fields. Immediately after cardiac arrest La was present as precipitates only in a few myocytes adjacent to the outer mitochondrial membrane as seen by cTEM and ESI. In such cells La was also detected by EELS in mitochondrial matrix and myofibrils. Advanced ischemic stress led to an increase of the percentage of myocytes containing detectable intracellular La. After 45 min ischemia at 30 degrees C, myocytes and mitochondria showed a remarkable ed...Continue Reading

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Citations

Dec 4, 2003·Bioelectrochemistry·M SchaeferM M Gebhard
Jun 26, 2018·Chemistry : a European Journal·Riikka Arppe-TabbaraThomas Just Sørensen
May 30, 2003·American Journal of Physiology. Cell Physiology·John P Reeves, Madalina Condrescu

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