Cellular Dynamics and Genomic Identity of Centromeres in Cereal Blast Fungus

MBio
Vikas YadavNaweed I Naqvi

Abstract

Precise kinetochore-microtubule interactions ensure faithful chromosome segregation in eukaryotes. Centromeres, identified as scaffolding sites for kinetochore assembly, are among the most rapidly evolving chromosomal loci in terms of the DNA sequence and length and organization of intrinsic elements. Neither the centromere structure nor the kinetochore dynamics is well studied in plant-pathogenic fungi. Here, we sought to understand the process of chromosome segregation in the rice blast fungus Magnaporthe oryzae High-resolution imaging of green fluorescent protein (GFP)-tagged inner kinetochore proteins CenpA and CenpC revealed unusual albeit transient declustering of centromeres just before anaphase separation of chromosomes in M. oryzae Strikingly, the declustered centromeres positioned randomly at the spindle midzone without an apparent metaphase plate per se Using CenpA chromatin immunoprecipitation followed by deep sequencing, all seven centromeres in M. oryzae were found to be regional, spanning 57-kb to 109-kb transcriptionally poor regions. Highly AT-rich and heavily methylated DNA sequences were the only common defining features of all the centromeres in rice blast. Lack of centromere-specific DNA sequence motifs or ...Continue Reading

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Citations

May 6, 2020·The New Phytologist·Qing ShenNaweed I Naqvi
Jul 25, 2020·Annual Review of Microbiology·Krishnendu GuinKaustuv Sanyal
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Sep 28, 2020·Fungal Genetics and Biology : FG & B·Mariel A Pfeifer, Chang Hyun Khang

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Datasets Mentioned

BETA
FJ81278
PRJNA504461

Methods Mentioned

BETA
immunoprecipitation
ChIP-seq
PCR
RNA-seq
scraping
electron microscopy
ChIP

Software Mentioned

Burrows Aligner ( BWA
BLAST
MetaMorph Premier
Fusion
GraphPad Prism
Adobe Photoshop
Geneious
Fiji
Easyfig
BLASTn

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